Phosphatidylserine(PS)istranslocatedfromtheinnertotheoutersurfaceofthecellforphagocyticcellrecognition.Thehumananticoagulant,AnnexinV,isa35kDaCa2-dependentphospholipidproteinwithahighaffinityforPS.AnnexinVlabeledwithCF™488A(Abs/Em=490/515nm)canidentifyapoptoticcellsingreenbybindingtoPSexposedontheouterleaflet.OurCF™488Adyeissuperiortofluorescein/FITCasitisnotaffectedbypHandhasmuchbetterphotostABIlity.Propidiumiodide(PI)isimpermeanttolivecellsorearlyapoptoticcells,butstainsnecroticcellsandlateapoptoticcellswithredfluorescence(Abs/EmwithDNA=535/617nm).BothPIandAnnexinV-CF™488Acanbeexcitedusingthe488nmlineofaflowcytometer.
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SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
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vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn