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Biotium/CF488A Annexin V and PI Apoptosis Kit/30061/100-assays

ThiskitcontainsCF488A-AnnexinVtostainapoptoticcellsandPItostainnecroticcells.

Phosphatidylserine(PS)istranslocatedfromtheinnertotheoutersurfaceofthecellforphagocyticcellrecognition.Thehumananticoagulant,AnnexinV,isa35kDaCa2-dependentphospholipidproteinwithahighaffinityforPS.AnnexinVlabeledwithCF™488A(Abs/Em=490/515nm)canidentifyapoptoticcellsingreenbybindingtoPSexposedontheouterleaflet.OurCF™488Adyeissuperiortofluorescein/FITCasitisnotaffectedbypHandhasmuchbetterphotostABIlity.Propidiumiodide(PI)isimpermeanttolivecellsorearlyapoptoticcells,butstainsnecroticcellsandlateapoptoticcellswithredfluorescence(Abs/EmwithDNA=535/617nm).BothPIandAnnexinV-CF™488Acanbeexcitedusingthe488nmlineofaflowcytometer.

References

1.CellDeathandDisease(2014),doi:10.1038/cddis.2013.550

2.Autophagy(2016),doi:10.1080/15548627.2016.1147667

3.JYoungPharm.(2016),doi:10.5530/jyp.2016.3.4

4.MolecularMedicineReports(2017),doi:10.3892/mmr.2017.6480

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