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Biotium/MCB Glutathione Detection Kit/30019/100-assays

MCBGlutathioneDetectionKitutilizesathiol-reactivedyemonochlorobimane(MCB),whichisessentiallynonfluorescentuntilitreactswithathioltoformabluefluorescentproduct.

Diminishedcellularglutathione(GSH)leveloccursattheearlystageofmitochondrion-associatedapoptosispathwayduetoGSHefflux.GSHdepletionfurtherleadstocytochromecreleaseandcaspase3induction(1,2).MCBGlutathioneDetectionKitutilizesathiol-reactivedyemonochlorobimane(MCB),whichisessentiallynonfluorescentuntilitreactswithathioltoformabluefluorescentproduct(λAbsEm=380/461nm).ByincubatingcelllysatewithMCB,theintensityofthefluorescentsignalgeneratedfromtheassayreflectstheamountofGSHpresentinthecells.Fluorescencecaneasilybedetectedusingafluorometeror96-wellfluorometricplatereaderwithapproximately1hourassaytime.

KitComponents:

  • Celllysisbuffer
  • Monochlorobimane(MCB)
  • GST enzyme

References

1.FASEBJ12(6),479(1998).

2.BiochemSocTrans28,56(2000).

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