NucView™405Caspase-3Substrate,1mMinDMSO,providesaconvenienttoolfordetectingapoptosisinintactcellsbasedoncaspase-3/7activityusingeitherconfocalmicroscopyorflowcytometry.
Incontrasttootherfluorogeniccaspasesubstratesorfluorescentcaspaseinhibitorbased(FLICA)assays,NucView™caspase-3substratescanbeusedtodetectcaspase-3/7activitywithinindividualintactcellswithoutinhibitingapoptosisprogression.NucView™substratesconsistofafluorogenicDNAdyecoupledtothecaspase-3/7DEVDrecognitionsequence.Thesubstrate,whichisinitiallynon-fluorescent,penetratestheplasmamembraneandentersthecytoplasm.Inapoptoticcells,caspase-3/7cleavesthesubstrate,releasingthehigh-affinityDNAdye,whichmigratestothecellnucleusandstainsDNAwithfluorescence.Thus,NucViewcaspase-3substratesarebifunctional,allowingdetectionofcaspase-3/7activityandvisualizationofmorphologicalchangesinthenucleusduringapoptosis.Thestainingisalsoformaldehyde-fixable.
NucView™405 dyeisexcitedbythe405nmlaserlinefordetectionbyconfocalmicroscopyintheDAPIchannel,orbyflowcytometryinthePacificBlue®channel.NucView™405isidealforcaspase-3detectioninmulti-colorapplicationsforresearcherswhowishtoreservethegreenfluorescencechannelforotherdetectionreagents.
Note:NucView™405signalmaybedifficulttoobserveusingepifluorescencemicroscopy.Confocalmicroscopyusing405nmlaserexcitationisrecommendedforimaging.
NucView™405isalsoavailableat1mMinPBS(catalogno.10407),foruseinDMSO-sensitivecells.Biotiumalsooffersgreen NucView™488caspase-3substrateandkitsaswellasorangefluorescentNucView™530.
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SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn