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Sino Biological/IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag/1/HG10856-NF

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IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: General Information

Gene
Species
Human
NCBI Ref Seq
NM_000634.2
RefSeq ORF Size
1053 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human interleukin 8 receptor, alpha with N terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-N-FLAG
Restriction Sites
KpnI + XbaI (6kb + 1.09kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

IL8Ra/CXCR1/CD181 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: Validated Images

Human aFGF/FGF1 Gene Plasmid Map 5637
Human IL8Ra/CXCR1/CD181 Gene Expression validated Image 16461
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: Synonyms

C-C cDNA ORF Clone, Human; C-C-CKR-1 cDNA ORF Clone, Human; CD128 cDNA ORF Clone, Human; CD181 cDNA ORF Clone, Human; CDw128a cDNA ORF Clone, Human; CKR-1 cDNA ORF Clone, Human; CMKAR1 cDNA ORF Clone, Human; IL8R1 cDNA ORF Clone, Human; IL8RA cDNA ORF Clone, Human; IL8RBA cDNA ORF Clone, Human

IL8Ra/CXCR1/CD181 Background Information

Full Name
chemokine (C-X-C motif) receptor 1
Research Areas
  • Neuroinflammation

IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human,Related Products

  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, N-GFPSpark® tagHG10856-ANG
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tagHG10856-CF
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, untaggedHG10856-UT
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, C-GFPSpark® tagHG10856-ACG
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, C-His tagHG10856-CH
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, C-HA tagHG10856-CY
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone in Cloning Vector, HumanHG10856-M
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, N-His tagHG10856-NH
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, N-Myc tagHG10856-NM
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, N-HA tagHG10856-NY
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, C-OFPSpark® tagHG10856-ACR
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Human, C-Myc tagHG10856-CM
  • IL8Ra/CXCR1/CD181 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tagHG10856-ACGLN
  • Human IL8Ra/CXCR1/CD181 qPCR Primer PairHP100751

IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse,Related Products

  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, N-His tagMG50851-NH
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  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, N-DYKDDDDK (Flag®) tagMG50851-NF
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, C-GFPSpark® tagMG50851-ACG
  • IL8Ra/CXCR1/CD181 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tagMG50851-ACGLN
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone in Cloning Vector, MouseMG50851-G
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, N-Myc tagMG50851-NM
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, untaggedMG50851-UT
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, C-Myc tagMG50851-CM
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, C-HA tagMG50851-CY
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tagMG50851-CF
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, C-His tagMG50851-CH
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Mouse, N-HA tagMG50851-NY
  • Mouse IL8Ra/CXCR1/CD181 qPCR Primer PairMP200822

IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat,Related Products

  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat, N-His tagRG80202-NH
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  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat, C-Myc tagRG80202-CM
  • IL8Ra/CXCR1/CD181 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tagRG80202-ACGLN
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat, untaggedRG80202-UT
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat, C-OFPSpark® tagRG80202-ACR
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat, C-DYKDDDDK (Flag®) tagRG80202-CF
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone in Cloning Vector, RatRG80202-G
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat, N-HA tagRG80202-NY
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  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat, N-DYKDDDDK (Flag®) tagRG80202-NF
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat, N-Myc tagRG80202-NM
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rat, C-His tagRG80202-CH
  • Rat IL8Ra/CXCR1/CD181 qPCR Primer PairRP300194

IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rhesus,Related Products

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  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rhesus, C-Myc tagCG90117-CM
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rhesus, N-DYKDDDDK (Flag®) tagCG90117-NF
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rhesus, N-Myc tagCG90117-NM
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rhesus, untaggedCG90117-UT
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rhesus, C-HA tagCG90117-CY
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone in Cloning Vector, RhesusCG90117-G
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rhesus, N-HA tagCG90117-NY
  • IL8Ra/CXCR1/CD181 Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tagCG90117-ACGLN
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rhesus, C-GFPSpark® tagCG90117-ACG
  • IL8Ra/CXCR1/CD181 cDNA ORF Clone, Rhesus, C-DYKDDDDK (Flag®) tagCG90117-CF
  • Mesothelial Cell HIF1α Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk
    Author
    Hart, PC;Kenny, HA;Grassl, N;Watters, KM;Litchfield, LM;Coscia, F;Blaženović, I;Ploetzky, L;Fiehn, O;Mann, M;Lengyel, E;Romero, IL;
    Year
    2019
    Journal
    Cell Rep
    Application
    template
    PubMed ID: 31851935FoldedExpand

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