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ImmunoVision/DNA-3000/1 mg/DNA-3000

Product Name:     DNA (Double Stranded) from Plasmid

Description:

The dsDNA antigen is a 2690 bp plasmid purified by alkaline lysis and chromatography. The purification process includes operations to minimize reactivity to antibodies against single-stranded DNA. Use of plasmid DNA in ELISA is an effective method for the detection of anti-double-stranded (ds) DNA antibodies (1). The presence of antibodies to dsDNA is included in the 1982 revised criteria for classification of Systemic Lupus Erythematosus (SLE) (2). Approximately 60-70% of SLE patients have antibodies to dsDNA, and there is considerable evidence to implicate immune complexes containing anti-dsDNA and DNA in the pathogenesis of SLE (3,4). In addition, levels of anti-dsDNA antibodies have been demonstrated to correlate with disease activity in many patients (5,6). Low levels of anti-dsDNA antibodies may occur in other rheumatic diseases (7) and may occur at a very low frequency (<2%) in individuals without any symptoms of rheumatic disease (8). Anti-dsDNA antibodies may appear in rheumatic patients prior to the development of disease manifestations (9).

CodeAmountPriceInquiry
DNA-3000
1 mg
$1500.00
DNA-3250
250 microgram
$550.00

References:

  1. Emlen W, et al. J.Immunol. Methods Vol 132:pg 91-101 (1990).
  2. Tan EM, et al. Arthrits Rheum Vol 25:pg 1271-1277 (1982).
  3. Nakamura RM, Peebles CL, Molden DP, Tan, EM. Lab. Medicine Vol 15: pg 190-198 (1984).
  4. Condemi JJ. JAMA Vol 258: pg 2920-2929 (1987).
  5. Tan EM, Schuer PH, Carr RI, Kunkel HG. J Clin Invest Vol 45: pg 1732-1740 (1966).
  6. Miniter MF, Stollar BD, Agnello V. Arthritis Rheum Vol 22: pg 959-968 (1979).
  7. Shoenfeld Y, Andre-Schwartz A, Stollar BD, Schwartz S. in Systemic Lupus Erythematosus, John Wiley & Sons, pg 213-255 (1987).
  8. Stollar BD. Clin. Immun. Allergy Vol 1: pg 243-260 (1981).
  9. Swaak AJG, et al. Annals of Rheum Dis Vol 41: pg 388-395 (1982).

Specifications:

  • DNA-3000

  • DNA-3250

Certificate of Analysis:

  • 5745

  • 5817

  • 6132

  • 6185

  • 6191

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