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产品资料

alfa/43318Brilliant Blue G, ultrapure/50g/43318.18

Brilliant Blue G, ultrapure

MDL
MFCD00078482
EINECS
228-058-4

化学性质

化学式
C47H48N3NaO7S2
式量
854.04
表格
Powder
Storage & Sensitivity
Ambient temperatures.
可溶性
Soluble in water (50 mg/ml) and ethanol (40 mg/ml).

应用

Brilliant Blue G is used in analytical biochemistry dye and as a P2X7 antagonist. It is also used in protein staining following gel electrophoresis. It is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery. It is useful for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents.

注释

E(m) (595nm, H{2}O) >36,300

参考文献

Hiroshi Enaida.; Toshio Hisatomi.; Yasuaki Hata.; Akifumi Ueno.; Yoshinobu Goto.; Tomomi Yamada.; Toshiaki Kubota.; Tatsuro Ishibashi. Brilliant blue G selectively stains the internal limiting membrane/brilliant blue G-assisted membrane peeling. Retina. 2006, 26, (6), 631-636.

John Pierce.; C.H. Suelter. An evaluation of the Coomassie brilliant blue G-250 dye-binding method for quantitative protein determination. Analytical Biochemistry. 1977, 81 (2), 478-480.

其他参考资料

协调关税代码
3204.12
有毒物质控制法
Yes

文件

产品 SDS

质量分析报告(COA)

产品说明书

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