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| Clone: | S12-I |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | SyntheticpeptidecorrespondingtoaC-terminalsequenceofhumanβ-actin. |
| UniProtID: | P60709(β-actin) |
| Speciesreactivity: | Human,Mouse,Rat |
| Specificity: | Recognizesα-actinandβ-actin. |
| Applications: | ELISA,IP,WB |
| RecommendedDilutions/Conditions: | ELISA(1:10,000-1:20,000) WesternBlot(1:2,000;Washbuffer:1xTRISbufferedsaline(TBS)containing0.1%TritonX-100,Blockingbuffer:1xTBScontaining0.1%TritonX-100and5%nonfatdrymilk.Incubatethemembranewithantibodydilutedinblockingbufferfor2hoursatroomtemperature) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| PurityDetail: | MajorcloneofIgGsobtainedfromimmunoaffinitypurifiedimmunoglobulinscorrespondingtoimmunogenicpeptide. |
| Formulation: | Liquid.In20mMTRIS/HCl,pH8.0,containing10mg/mlBSAand0.05%sodiumazide. |
| Use/StABIlity: | Stableforatleast12monthsafterreceiptwhenstoredasrecommended. |
| Handling: | Avoidfreeze/thawcycles. |
| Shipping: | BlueIceNotFrozen |
| LongTermStorage: | +4°C |
| TechnicalInfo/ProductNotes: | ClonalRabbitAntibodyisapurehomogenousfractionofrabbitimmunoglobulin(IgG)whichcorrespondstoauniquelinearepitope onthetargetprotein.Thisepitopeisdesignedafteradetailedanalysisoftheantigenstructure,regulatorypost-translationalmodifications,protein/proteininteractiondomainandproteinfolding.Thechosensequence(peptide)issynthesizedandtherabbitisimmunized.Thecloneisthenseparatedfromthecrudeantiserumby "invitrocloningtechnology".Theresultingproductismonospecificandcharacterizedbysuperiorspecificity,affinityandAvidity. |
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