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| Clone: | ARM193 |
| Host: | Mouse |
| Isotype: | IgG2b |
| Immunogen: | E. coli RecA. |
| UniProt ID: | P0A7G6 |
| Species reactivity: | E. coli |
| Applications: | WB Inhibition assay |
| Recommended Dilutions/Conditions: | Western Blot (1µg/ml, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Inhibition Assay (Equal to RecA protein). Detects a band of ~40kDa by Western blot. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Lyophilized. In PBS, pH 7.2, containing 1% sucrose and 0.09% sodium azide. For a stock solution of 1.0mg/ml reconstitute with 100μl of sterile distilled water. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | The RecA protein is a member of the RecA/Rad51 family of proteins which are ubiquitous among organisms from bacteria to humans. RecA protein of E. coli is a catalyst of DNA strand exchange in the early phase of homologous recombination involving the exchange of two aligned, nearly identical strands of DNA. RecA is also a key enzyme in recombinational repair of damaged DNA. |
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