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Expressionsystems/BestBac™ Linearized Baculovirus DNA/91-100/1 Ea

BestBac1.0BaculovirusCotransfectionKit

ExpressionSystems’popularBestBacLinearizedBaculovirusDNAbackbonehasbeenengineeredtodeliverhighlyefficientrecombinationwithallpolyhedrongenelocusbasedtransfervectors.TheresultingvirusvectorprovidessuperiorstABIlityandpuritycomparedtoothersystems.BestBacisavailablewithadeletionorwild-typeattheV-cath/chiAregionandsoldindividuallyorinkits.

Description:

  • UnmatchedproductivityandstabilityforBEVS
  • Improvedproductionmethodologycanreduceneedforplaquepurification
  • Produceshightitersof5E8to3E9IU/mlofrecombinantbaculovirusinP1stock
  • GeneratesP1stockasquicklyasBac-to-Bac®andothersystems
  • EachBestBacvialcontainsenoughmaterialforaminimumoffivecotransfections
  • SuitableforotherexpressionsystemsincludingBacMamTM

AvailableinTwoVersions

  • BestBac1.0iswildtypeatthecathepsinandchitinase(v-cath/chiA)loci,preferredforsomeapplications.
  • BestBac2.0isadeletionmutantofBestBac1.0.Thev-cath/chiAdeletionremovesapotentiallydetrimentalproteaseandmayreducecompetitionforresourcesforproteinsynthesisduringlategeneexpression.Thev-cath/chiAdeletioncanimprovetheintegrityofyourexpressedproteinresultinginasignificantincreaseoffunctionalproteinyield.

AlsoAvailableinaCotransfectionKit

Containseverythingyouneedtoperformcotransfectionswithyourtransfervector.Includedinthekitare:

  • BestBacDNA
  • pVL1393-lacZcontrolvector
  • Transfectionmedium
  • Expres2TRtransfectionreagent

Specification:

MediatypeLinearizedBaculovirusDNA
Shippingcondition2–8⁰C
Storagecondition2-8⁰C,protectfromlight
Use-bydateOneyearfromdateofmanufacture


RecommendedStorageCondition

2–8ºC
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