Antibodies | |
FullName | Donkeyanti-ChickenIgYFcBeads |
Host | Donkey |
Quantity | 1.0mlbedvolume |
Volume | 1mlsettledgel |
Type | ImmunoprecipitationGel |
PhysicalState | Gel |
Concentration | N/A |
Diluent | Phosphatebufferedsaline‚pH7.2 |
Preservative | 0.075%sodiumazide |
Procedure | AdonkeywasimmunizedwithpurifiedchickenIgY.Anti-IgYFcwasisolatedfromimmuneserausingaffinitychromatographyandconjugatedtoN-hydroxysuccinimide(NHS)-activatedagarosebeads.BeadswerewashedextensivelyaftertheblockingoftheresidualNHSsites. |
Specifications | Thespecificactivityoftheanti-IgY-agarosewasdeterminedbyapplying2.7mgIgYin24mlPBS(0.1mg/ml)to1.0mlbedvolumeofagarose.Twomlaliquotswerecollected.ThetotalamountofIgYthatboundtothecolumnwascalculated.Forthislot,1.3mgIgYboundpermlagarose. |
Storage | 4°C |
Note | Forinvitrodiagnostic‚researchorproteomicsapplicationsonly. |
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