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MACS/StemMACS CHIR99021/130-103-926/

Content:2mg

Overview

StemMACS™CHIR99021isaselectivesmallmoleculeGSK3inhibitorthatactivatesWntsignaling.

Details

Backgroundinformation

StemMACS™CHIR99021isahighlyselectiveinhibitorofglycogensynthasekinase3(GSK-3),acrucialregulatoroftheWntsignalingpathway.TheaminopyridineCHIR99021inhibitsbothGSK-3isoforms,GSK-3α(IC5010nM)andGSK-3β(IC506.7nM).UnlikeotherGSK-3inhibitors,itdoesnotcross-reactwithcyclin-dependentkinases(CDKs).ActivationofWntsignalingviaCHIR99021-mediatedGSK-3inhibitioniswidelyusedtomodulatepluripotentstemcelldifferentiationandself-renewal.

Applications

PublishedapplicationsforCHIR99021include:
  • Naïve,groundstatecultureofmurineESandiPScellsusing2iconditions1
  • Naïve,groundstatecultureofratESandiPScellsusing3iconditions2
  • Naïve,groundstatecultureofhumanESandiPScells3
  • DifferentiationofhumanESandiPScellsintocardiomyocytes4
  • DifferentiationofhumanESandiPScellsintoskeletalmuscle5
  • DifferentiationofhumanESandiPScellsintodefinitiveendoderm6
  • ChemicalReprogrammingofmousefibroblasts5
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