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MACS/Neurosphere Dissociation Kit (P)/130-095-943/

Capacity:for100preparations

Overview

TheNeurosphereDissociationKitshavebeendevelopedforthegentleandefficientgenerationofsingle-cellsUSPensionsfromculturedneurospheres.Inlessthananhour,thegentleenzymaticdissociationprocedureefficientlyyieldshighnumbersofviablecells.
Dissociatedcellsareimmediatelyreadyforfurtherinvitroorinvivoapplicationssuchascellcultureandexpansion,flowcytometry,fluorescencemicroscopy,andmolecularapplications,orforcellseparationsusingMACS®Technology.

Details

Backgroundinformation

Neurospheresarenon-adherentsphericalclustersofcellsformedwhenneuralstemcellsareculturedunderappropriateconditions.Effectivedissociationisimportantforeffectivepasagingandsubsequentdownstreamapplications
Someantigenepitopesaredegradedbypapain,othersbytrypsin.ThekitbasedonpapainisbestsuitedforusewithAnti-Prominin-1MicroBeads,CD11b(Microglia)MicroBeadsandAnti-A2B5MicroBeads,whereas,forexample,Anti-GLAST(ACSA-1)MicroBeadKitandAnti-PSA-NCAMMicroBeadsrequiretheuseofthetrypsin-basedkit.
PleaseseeFigure2forfurtherinformationwithregardtoepitopesensitivityandkitselection.
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