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Maintenance of Probes in bacteria including Escherichia coli188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Maintenance of Probes in bacteria including Escherichia coli

Plasmid(pUCseries)containinggenomicDNAfragmentsaremaintainedinE.colistrainDH5aTM.TheE.coliculturesareroutinelyculturedat37ConLuria-Bertani(LB)agaronorinLBbrothcontainingAmpicillin(30µg/ml)orCarbenicillin(50µg/mlbroth,100µg/mlagar).E.colistrainsareusuallypreservedinstabagarorglycerolformid-termstorageandlyophilizedforlong-termstorage.

StorageinStabAgar:MoststrainsofE.colicanbestoredforonetotwoyearsinstabagar.

  • Useairtight,autoclavedvialscontaining2/3full(appr.1ml)ofmedium.

  • Inoculateeachvialwithasinglecolony.

  • Leavethecapofthevialsightlylooseandincubateovernightat37C.

  • Sealthevialtightlyandstoredatroomtemperatureinadarkplace.

  • Toreviveastoredstrain,streakontoLBplatecontainingappropriateantibioticsforasinglecolony.

    • StorageinglycerolSolution:MoststrainsofbacteriaincludingE.coli,andfungi(sporesispreferred)canbestoredforonetotwoyearsinglycerolsolutionat-20C.At-70Ctheycanbestoredalmostforlifetime.

  • Prepare0.5mlofglycerolsolutionin2.0mlscrew-capvials,autoclave(15min,121C),cool,andstorethematroomtemperature.

  • Transferonedrop(0.5ml)ofanovernight-growncultureintoavial.

  • Voltex,storeat-20or-70C(-70Cstorageispreferredforlong-termmaintenance).

  • Toreviveastoredstrain,streakontoLBplatecontainingappropriateantibioticsforasinglecolony.


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