RFectsiRNA/miRNA转染试剂
Fig.1.RFectsRNAtransfectionreagentoffersthehighesttransfectionefficiency. HeLacellsweretransfectedwithAlexa546-dyelabeledsiRNAatfinalconcentrationof10nMusingRFectsRNA transfectionreagent.After24hrs,thecellswerestainedwithHoechst33342(nuclearstainingdye)andthenwereviewedbyfluorescencemicroscopy.AThefluorescenceimagesshowthefluorescenceoflabeledsiRNA.B.ThefluorescenceimagesshowthefluorescenceoflabeledsiRNA(inred)overlaidwithfluorescencefromHoechst33342nuclearstaining(inblue).
Fig.2.RFectsRNAtransfectionreagentoffershighlevelofgeneknockdownwithlowconcentrationofsiRNA.LaminA/CsiRNA-RFecttransfectioncomplexeswerepreparedin96-wellplates,andthenA549cellswereaddedtoeachwell.
Fig.3.RFectsRNAtransfectionreagentoffersthehighestlevelofgeneknockdownonavarietyofcelltypes.DifferentcelllinesweretransfectedwithscrambledorLaminA/CsiRNA(finalconcentration:10nM)usingRFect Inthisandallthefollowingfigures,allthecellsareharvested48hoursaftertransfectionandLaminA/CknockdownwasmeasuredbyqRT-PCR.相关文献
1.CellResearch.(2017)27.5-methylcytosinepromotesmRNAexport-NSUN2asthemethyltransferaseandALYREFasanm5Creader.(IF:14.812)
2.NucleicAcidsResearch.2015May12.XPDlocalizesinmitochondriaandprotectsthemitochondrialgenomefromoxidativeDNAdamage.(IF:9.202)
3.ScientificReports.2013Jan1.APG:anActiveProtein-GeneNetworkModeltoQuantifyRegulatorySignalsinComplexBiologicalSystems.(IF:5.078)
4.Oncotarget.2016.Knockdownofgolgiphosphoprotein2inhibitshepatocellularcarcinomacellproliferationandmotility.(IF:5.008)
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