Peerreviewed RPA publications | TwistDx

Every year more and more scientists are finding out that RPA really works. Over 350peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

Recombinase Polymerase Amplification Combined with Lateral Flow Strip for Listeria monocytogenes Detection in Food. Author: Du X-J, Zang Y-X, Liu H-B, Li P, Wang S. Experiments confirmed a detection limit as low as 300 fg of DNA and 1.5 × 101 CFU in pure cultures. Furthermore, RPA‐LF exhibited no cross‐reactions with pathogens. Evaluation of the method with food samples indicated that the detection limit was ... Read more
Detection of Entamoeba Species: A Comparative Analysis of Nested Multiplex PCR and Recombinase Polymerase Amplification Author: Philips SA, Manochitra K, Padukone S, Chandra S. Parija. The aim of the present study was to compare the diagnostic ability of nested-multiplex PCR and Recombinase polymerase amplification for differential detection of E. histolytica and E. dispar. The results of this study showed good agreement between the two tests. ... Read more
An isothermal based recombinase polymerase amplification assay for rapid, sensitive and robust indexing of citrus yellow mosaic virus. Author: Kumar PV, Sharma SK, Rishi N, Ghosh DK, Baranwal VK. The results from the present study indicated that RPA assay can be used easily in routine indexing of citrus planting material. To the best of our knowledge, this is the first report on development of a rapid and simplified isothermal ... Read more
Rapid Detection of Staphylococcus aureus via Recombinase Polymerase Amplification Combined with Lateral Flow Strip Author: Du X-J, Zang Y-X, Liu H-B, Li P, Wang S. Under optimal conditions of 10 min at 40 °C for RPA, followed by 5 min at room temperature for LF, this method could detect 500 fg genomic DNA and 1.2 × 101 CFU/mL of S. aureus in pure culture. Specificity analysis showed ... Read more
Development of an isothermal amplification-based assay for the rapid detection of Cronobacter spp. Author: Liu S, Geng Y, Liu L, Sun X, Shao J, Han B, Wang J, Tan K. The RPA and real-time RPA assays reduced the analysis time to less than 15 min and the results were as reliable as those of real-time PCR. Taken together, the RPA and real-time RPA assays served as fast, reliable, and sensitive ... Read more
Rapid and sensitive detection of mink circovirus by recombinase polymerase amplification Author: Ge J, Shi Y, Cui X, Gu S, Zhao L, Chen H. In this study, we developed a nested PCR and established a novel recombinase polymerase amplification (RPA) assay for MiCV detection. Sensitivity analysis showed that the detection limit of nested PCR and RPA assay was 101 copies/reaction, and these methods were ... Read more
Diagnosis of Tick-Borne Illnesses by Use of One-Step Isothermal Nucleic Acid Amplification and Bio-Optical Sensor Detection Author: Kim JY, Koo B, Jin CE, Kim MC, Chong YP, Lee S-O, Choi S-H, Kim YS, Woo JH, Shin Y, Kim S-H. The clinical sensitivity of iNAD (100%; 95% CI, 83-100) for SFTS was significantly higher than that of real-time PCR (75%; 95% CI, 51-91; P = 0.047), while its clinical specificity (86%; 95% CI, 65-97) was similar to that of real-time ... Read more
Rapid and visual detection of Coxiella burnetii using recombinase polymerase amplification combined with lateral flow strips Author: Qi Y, Yin Q, Shao Y, Li S, Chen H, Shen W, Rao J, Li J, Li X, Sun Y, Lin Y, Deng Y, Zeng W, Zheng S, Liu S, Li Y. The detection system has a good specificity for detection of C. burnetii xinqiao 18 strain without cross-reaction with R.rickettsii, R.heilongjiangensis, R.sibirica, O.19 tsutsugamushi, S.aureus, orS.suis. It can detect recombinant 23SrRNA-pUC1920 plasmid and genomic DNA at levels of 10 copies/reaction and ... Read more
First report of Pythium root rot caused by Pythium mastophorum on parsley in the United States Author: Tsuchida C, Mauzey S, Hatlen R, Miles TD, Koike ST. We believe this is the first report of P. mastophorum infecting parsley in California. P. mastophorum has been reported on parsley only in southeastern Australia (Petkowski et al., 2013). In California, this species has been reported on celery (Vazquez et ... Read more
A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitides Author: Rivas L, Reutersward P, Rasti R, Herrmann B, Martensson A, Alfven T, Gantelius J, H Andersson-Svahn. In this article, we describe a colorimetric vertical-flow DNA microarray (DNA-VFM) that takes advantage of the screening capability of DNA microarrays in a paper format together with isothermal amplification by means of Recombinase Polymerase Amplification (RPA). Read more