部分文献列表gentleMACS™组织处理器 人来源样品膀胱活组织检测样品中提取RNA:Gamper,M.etal.(2009)Geneexpressionprofileofbladdertissueofpatientswithulcerativeinterstitialcystitis.BMCGenomics10:199*.
骨髓抽提物分选杜氏利什曼原虫:Kindt,R.etal.(2010)Towardsanunbiasedmetabolicprofilingofprotozoanparasites:optimisationofaLeishmaniasamplingprotocolforHILIC-orbitrapanalysis.Analyticaland BioanalyticalChemistry DOI10.1007/s00216-010-4139-0*.
蜕膜组织分选CD4+T细胞:Vacca,P.etal.(2010)CrosstalkbetweendecidualNKandCD14+myelomonocyticcellsresultsininductionofTregsandimmunosuppression.PNAS107:11918–11923.
拟胚体ES/iPS细胞解离:Niwa,A.etal.(2011)ANovelSerum-FreeMonolayerCultureforOrderlyHematopoieticDifferentiationofHumanPluripotentCellsviaMesodermal
Progenitors.PLoSone6:e22261.
粘膜分选固有层单核细胞:Isolationlaminapropriamononuclearcells:Geremia,A.etal.(2011)IL-23–responsiveinnatelymphoidcellsareincreasedininflammatoryboweldisease.JournalofCell
BIOLOGy.Epubaheadofprint.
鼻粘膜组织分选巨噬细胞:Krysko,O.etal.(2010)AlternativelyactivatedmacrophagesandimpairedphagocytosisofS.aureusinchronicrhinosinusitis.ExperimentalAllergyandImmunology.66:396–403.
胎盘从胎盘组织匀浆中提取脱氧雪腐镰刀菌烯醇:Nielsen,J.etal.(2011)Deoxynivalenoltransportacrossthehumanplacentalbarrier.FoodandChemicalToxicology 49:2046–2052.
皮肤分选CD4+T细胞:Reefer,A.etal.(2010)FlowcytometryimagingidentifiesrareTH2cellsexpressingthymicstromallymphopoietinreceptorina‘‘proallergic’’milieu.TheJournalofAllergy andClinicalImmunology126:1049–1058.
粪便提取细菌DNA:Walker,A.etal.(2009)Dominantanddiet-responsivegroupsofbacteriawithinthehumancolonicmicrobiota.TheISMEJournal5:220–230*.
滑膜分选滑膜液单核细胞:Sattler,A.etal.(2009)Cytokine-inducedhumanIFN-γ–secretingeffector-memoryThcellsinchronicautoimmuneinflammation.Blood113:1948–1956.
胸腺分选胸腺基质细胞和胸腺上皮细胞:WoodsIgnatoski,K.etal.(2011)DirectedTrans-DifferentiationofThymusCellsintoParathyroid-LikeCellsWithoutGeneticManipulation.TissueEngineeringDOI:10.1089/ten.tec.2011.0170.
扁桃体分选扁桃体单核细胞:Moroso,V.etal.(2011)NKcellscangeneratefromprecursorintheadulthumanliver.Blood. Epubaheadofprint
肿瘤从卵巢癌组织中分选上皮细胞和间质细胞:Strauss,R.etal.(2010)AnalysisofEpithelialandMesenchymal
MarkersinOvarianCancerRevealsPhenotypicHeterogeneityandPlasticity.PLoSone6:e1618
从肝细胞肿瘤中分选肝浸润淋巴细胞:Zhi-QingYangetal.(2010)Increasedliver-infiltratingCD8+FoxP3+regulatoryTcellsareassociatedwithtumorstageinhepatocellularcarcinomapatients.HumanImmunology71:1180–1186.
口腔疣状瘤中制备核悬液:Pentenero,M.etal.(2010)Distinctivechromosomalinst
ABIlitypatternsinoralverrucousandsquamouscellcarcinomasdetectedbyhigh-resolutionDNAflow
cytometry.CancerDOI:10.1002/cncr.26126.
从视网膜瘤中提取RNA:Benhammou,J.etal.(2011)IdentificationofintragenicdeletionsandduplicationinFLCNgeneinBirt-Hogg-Dubsyndrome. Genes,Chromosomes&Cancer50:466–477.
从卵巢癌中提取RNA:Smerdel,M.etal.(2011)VEGFintheDevelopmentofOvarMalignancy.ClinicalOvarienCancer4:19–25*.
阴道组织分选CD4+T细胞和巨噬细胞:Wheeler,L.etal.(2011)InhibitionofHIVtransmissioninhumancervicovaginalexplantsandhumanizedmiceusingCD4aptamer-siRNAchimeras.JournalofClinicalInvestigation121:2401–2412.
小鼠样品脑分选单核细胞:Wisor,J.etal.(2011)Cerebralmicrogliamediatesleep/wakeandneuroinflammatoryeffectsofmethamphetamine.Brain,Behavior,andImmunity25:767–776.
Hu,Y.etal.(2010)IL-17RCIsRequiredforIL-17A–andIL-17F–DependentSignalingandthePathogenesisofExperimentalAutoimmuneEncephalomyelitis.JournalofImmunology184:4307–4316.
蛋白质提取:Schmidt,M.etal.(2010)IndividualStressVulnerabilityIsPredictedbyShort-TermMemoryandAMPAReceptorSubunitRatiointheHippocampus.TheJournalofNeuroscience30:16949–16958*.
Heinzmann,J.etal.(2010)IntrahippocampalCorticosteroneResponseinMiceSelectivelyBredforExtremesinStressReactivity:AMicrodialysisStudy. JournalofNeuroendocrinology22:1187–1197*.
结肠检测鼠伤寒沙门氏菌细菌载量:Varol,C.etal.(2009)IntestinalLaminaPropriaDendriticCellSubsetsHaveDifferentOriginandFunctions.Immunity31:502–512*.
耳朵提取DNA:Minassian,A.etal.(2011)PreclinicalDevelopmentofanInVivoBCGChallengeModelforTestingCandidateTBVaccineEfficacy.PLoSone6:e19840*.
拟胚体解离拟胚体:Schaedlich,K.etal.(2010)AsimplemethodtosortESC-derived
ADIpocytes.Cytometry77A:990–995.
心脏分选DC细胞:Cuervo,H.etal.(2011)Myeloid-DerivedSuppressorCellsInfiltratetheHeartinAcuteTrypanosomacruziInfection.JournalofImmunology.Epubaheadofprint.
肾脏分选肾小管:Yang,L.etal.(2010)EpithelialcellcyclearrestinG2/Mmediateskidneyfibrosisafterinjury.NatureMedicine16:535–544.
检测金黄色葡萄球菌载量:Biswas,L.etal.(2009)RoleoftheTwin-ArginineTranslocationPathwayinStaphylococcus.JournalofBacteriology191:5921–5929*.
Chlebowicz,M.etal.(2010)RecombinationbetweenccrCGenesinaTypeV(5C2&5)StaphylococcalCassetteChromosomemec(SCCmec)ofStaphylococcusaureusST398LeadstoConversionfromMethicillinResistancetoMethicillinSusceptibilityInVivo.AntimicrobialAgentsandChemotherapyDOI:10.1128/AAC.00696-09*.
肝脏分选白细胞:Clarke,C.etal.(2010)CXCchemokinereceptor-1isexpressedbyhepatocytesandregulatesliverrecoveryafterhepaticischemia/reperfusioninjury.Hepatology53:
261–271.
Jiang,Q.etal.(2010)mTORKinaseInhibitorAZD8055EnhancestheImmunotherapeuticActivityofanAgo
NISTCD40AntibodyinCancerTreatment.CancerResearch71:4074–4084.
李斯特菌细菌载量测定:Janot,L.etal.(2008)CD14WorkswithToll-LikeReceptor2toContributetoRecognitionandControlofListeriamonocytogenesInfection.TheJournalofInfectiousDiseases198:115–124*.
金黄色葡糖球菌细菌载量检测:Sibbald,M.etal.(2010)SyntheticEffectsofsecGandsecY2MutationsonExoproteomeBiogenesisinStaphylococcusaureus.JournalofBacteriology192:3788–3800*.
蛋白质抽提:Guabiraba,R.etal.(2010)RoleoftheChemokineReceptorsCCR1,CCR2andCCR4inthePathogenesisofExperimentalDengueInfectioninMice.PLoSone5:e15680*.
肺脏分选髓系衍生抑制细胞(Myeloid-derivedsuppressorcell):Shina,P.etal.(2011)Myeloid-derivedsuppressorcellsexpressthedeathreceptorFasandapoptoseinresponsetoTcell–expressedFasL.Blood117:5381–5390.
分选A型流感病毒:Hong,D.etal.(2011)CationicLipid/DNAComplex-AdjuvantedInfluenzaAVirusVaccinationInducesRobustCross-ProtectiveImmunity.JournalofVirology84:
12691–12702.
分选CD3+T细胞,CD4+T细胞和CD8+T细胞:Ruckwardt,T.etal.(2010)ResponsesagainstaSu
BDominantCD8+TCellEpitopeProtectagainstImmunopathologyCausedbyaDominantEpitope.JournalofImmunology185:4673–4680.
Szymczak,W.andDeepe,G(2009)TheCCL7-CCL2-CCR2AxisRegulatesIL-4ProductioninLungsandFungalImmunity.JournalofImmunology183:1964–1974.
Tanne,A.etal.(2009)AmurineDC-SIGNhomologuecontributestoearlyhostdefenseagainstMycobacteriumtuberculosis.JournalofExperimentalMedicine206:2205–2220.
Bouabe,H.etal.(2011)NovelHighlySensitiveIL-10–β-LactamaseReporterMouseRevealsCellsoftheInnateImmuneSystemasSubstantialSourceofIL-10InVivo.JournalofImmunology.Epubaheadofprint.
分选CD4+CD25+T细胞:Malmhäll,Y.etal.(2010)ExpansionofCD4+CD25+andCD25-T-Bet,GATA-3,Foxp3andRORctCellsinAllergicInflammation,LocalLungDistributionandChemokineGeneExpression.PLoSone6:e19889.
分选中性粒细胞和巨噬细胞:Tschöp,J.etal.(2010)TheCannabinoidReceptor2IsCriticalfortheHostResponsetoSepsis.JournalofImmunology183:499–505.
Poole,J.etal.(2011)OrganicDustAugmentsNucleotide-BindingOligomerizationDomain(NOD2)ExpressionviaanNF-κBPathwaytoNegativelyRegulateInflammatoryResponses.AmericanJournalofPhysiology.Epubaheadofprint.
分选DC细胞:Hasegawa,H.etal.(2011)Expressionsofmultidrugresistanceprotein1andmultidrugresistance-associatedprotein1inlungdendriticcells.LifeSciences89:282–289.
Dorr,A.etal.(2011)SourcesofalveolarsolubleTNFreceptorsduringacutelunginjuryofdifferentetiologies.JournalofAppliedPhysiology111:177–184.
Conenello,G.etal.(2010)ASingleN66SMutationinthePB1-F2ProteinofInfluenzaAVirusIncreasesVirulencebyInhibitingtheEarlyInterferonResponseInVivo.JournalofVirology85:652–662.
分选成纤维细胞:Roybal,J.etal.(2011)miR-200InhibitsLungAdenocarcinomaCellInvasionandMetastasisbyTargetinFlt1/VEGFR1.MolecularCancerResearch9:25–35.
Kilic,A.etal.(2011)NervegrowthfactorinducestypeIIIcollagenproductioninchronicallergicairwayinflammationJournalofAllergyandClinicalImmunology.Epubaheadofprint.
分选CD8+IFN-γ+T细胞:Bodewes,R.etal.(2010)RedundancyoftheinfluenzaAvirus-specificcytotoxicTlymphocyteresponseinHLA-B*2705transgenicmicelimitstheimpactofamutateintheimmunodominantNP383–391epitopeoninfluenzapathogenesis.VirusResearch155:123–130.
结核分歧杆菌细菌载量检测:Carlos,D.etal.(2008)HistaminePlaysanEssentialRegulatoryRoleinLungInflammationandProtectiveImmunityintheAcutePhaseofMycobacteriumtuberculosInfection.InfectionandImmunity77:5359–5368*.
检测绿脓杆菌细菌载量:Secher,T.etal.(2010)Anti-Pseudomonasaeruginosa
SEROtypeO11LPSimmunoglobulinMmonoclonalantibodypanobacumab(KBPA101)confersprotectioninamurinemodelofacutelunginfection.JournalofAntimicrobial
Chemotherapy66:1100–1109*.
提取RNA:Hermesh,T.etal.(2010)AntiviralInstructionofBoneMarroLeukocytesduringRespiratoryViralInfections.CellHost&Microbe7:343–353.
蛋白质提取:Fremond,C.etal.(2007)IL-1Receptor-MediatedSignalIsanEssentialComponentofMyD88-DependentInnateResponsetoMycobacteriumtuberculosisInfection.
JournalofImmunology179:1178–1189*.
Couillin,I.etal.(2009)IL-1R1/MyD88SignalingIsCriticalforElastase-InducedLungInflammationandEmphysemaJournalofImmunology183:8195–8202*.
淋巴结CD4+和CD8+T细胞分选:Verma,S.etal.(2010)SelenoproteinKKnockoutMiceExhibitDeficientCalciumFluxinImmuneCellsandImpairedImmuneResponses.JournalofImmunology186:
2127–2137.
提取RNA:Sigurdson,C.etal.(2008)BacterialColitisIncreasesSusceptibilitytoOralPrionDisease.TheJournalof InfectiousDiseases;199:243–252*.
粘膜分选DC细胞:Mascarell,L.etal.(2010)Oralmacrophage-likecellsplayakeyroleintoleranceinductionfollowingsublingualimmunotherapyofasthmaticmice.MucosalImmunology;Epubaheadofprint.
肌肉组织RNA提取:Erb,M.etal.(2009)OmigapilAmelioratesthePathologyofMuscleDystrophyCausedbyLaminin-2Deficiency.TheJournalofPharmacologyandExperimentalTherapeutics;531:787–795.
皮肤RNA提取:Wigglesworth,K.etal.(2011)RapidRecruitmentandActivationofMacrophagesbyAnti-Gal/α-GalLiposomeInteractionAcceleratesWoundHealing.Journalof
Immunology;186:4422–4432.
脊髓分选骨髓来源干细胞:Jamous,M.etal.(2010)PurificationofMouseBoneMarrow-DerivedStemCellsPromotesExVivoNeuronalDifferentiation.CellTransplantation;19:193–202.
分选单核细胞:Gültner,S.etal.(2010)ReducedTregfrequencyinLFA-1-deficientmiceallowsenhancedTeffectordifferentiationandpathologyinEAE.EuropeanJournalofImmunology;40:3403–3412.
Hu,Y.etal.(2010)IL-17RCIsRequiredforIL-17A–andIL-17F–DependentSignalingandthePathogenesisofExperimentalAutoimmuneEncephalomyelitis.JournalofImmunology;184:4307–4316.
分选脊髓细胞:Shechter,R.etal.(2009)InfiltratingBlood-DerivedMacrophagesAreVitalCellsPlayinganAnti-inflammatoryRoleinRecoveryfromSpinalCordInjuryinMice.
PLoSmedicine;6:e1000113*.
脾脏分选A型流感病毒:Hong,D.etal.(2011)CationicLipid/DNAComplex-AdjuvantedInfluenzaAVirusVaccinationInducesRobustCross-ProtectiveImmunity.JournalofVirology84: 12691–12702.
分选CD3+T细胞,CD4+T细胞和CD8+T细胞:Ruckwardt,T.etal.(2010)ResponsesagainstaSubdominantCD8+TCellEpitopeProtectagainstImmunopathologyCausedbyaDominantEpitope.JournalofImmunology185:4673–4680.
Bouabe,H.etal.(2011)NovelHighlySensitiveIL-10–β-LactamaseReporterMouseRevealsCellsoftheInnateImmuneSystemasSubstantialSourceofIL-10InVivo.
JournalofImmunology.Epubaheadofprint.
分选脾细胞:Leclercq,S.etal.(2011)5-aza-2-deoxycytidine/valproatecombinationinducesCTLresponseagainstmesothelioma.EuropeanRespiratoryJournal.Epubaheadofprint.
Zvi,A.etal.(2011)Whole-GenomeImmunoinformaticAnalysisofF.tularensis:PredictedCTLEpitopesClusteredinHotspotsArePronetoElicitaT-CellResponse.PLoSone6:e20050.
检测李斯特菌细菌载量:Janot,L.etal.(2008)CD14WorkswithToll-LikeReceptor2toContributetoRecognitionandControlofListeriamonocytogenesInfection.TheJournalofInfectiousDiseases198:115–124*.
提取RNA:Hermesh,T.etal.(2010)AntiviralInstructionofBoneMarrowLeukocytesduringRespiratoryViralInfections.CellHost&Microbe7:343–353.
提取蛋白:Guabiraba,R.etal.(2010)RoleoftheChemokineReceptorsCCR1,CCR2andCCR4inthePathogenesisofExperimentalDengueInfectioninMice.PLoSone5:e15680*.
胸腺分选胸腺上皮细胞:Williams,K.etal.(2009)SingleCellAnalysisofComplexThymusStromalCellPopulations:RapidThymicEpitheliaPreparationCharacterizesRadiationInjury.CTS2:279–285.
McLelland,B.etal.(2011)Enhancedisolationofadultthymicepithelialcellsubsetsformultiparameterflowcytometryandgeneexpressionanalysis.JournalofImmunologicalMethods367:85–94.
提取蛋白:Laan,M.etal.(2009)AutoimmuneRegulatorDeficiencyResultsinDecreasedExpressionofCCR4andCCR7LigandsandinDelayedMigrationofCD4+Thymocytes.JournalofImmunology183:7682–7691.
提取RNA:Hermesh,T.etal.(2010)AntiviralInstructionofBoneMarrowLeukocytesduringRespiratoryViralInfections.CellHost&Microbe7:343–353.
肿瘤从胰腺癌肿瘤中分选单核细胞:Tseng,W.etal.(2010DevelopmentofanOrthotopicModelofInvasivePancreaticCancerinanImmunocompetentMurineHost.ClinicalCancerResearch16:3684–3695.
Gnerlich,J.etal.(2010)InductionofTh17CellsintheTumorMicroenvironmentImprovesSurvivalinaMurineModelofPancreaticCancer.JournalofImmunology185:4063–4071.
从黑色素瘤组织中分选CD8+T细胞:Bellavance,E.etal.(2011)DevelopmentofTumor-InfiltratingCD8+TCellMemoryPrecursorEffectorCellsandAntimelanomaMemoryResponsesAretheResultofVaccinationandTGF-βBlockadeduringthePerioperativePeriodofTumorResection.JournalofImmunology186:3309–3316.
分选AK7肿瘤中分选CD8a+肿瘤浸润淋巴细胞:Leclercq,S.etal.(2011)5-aza-2-deoxycytidine/valproatecombinationinducesCTLresponseagainstmesothelioma.EuropeanRespiratoryJournal.Epubaheadofprint.
从皮下肿瘤组织分选DC细胞:Alfaro,C.etal.(2011)Carcinoma-DerivedInterleukin-8DisorientsDendriticCellMigrationWithoutImpairingT-CellStimulation.PLoSone6:e17922.
从皮下肿瘤组织中分选CD8+,CD4+FoxP3-和CD4+FoxP3+T细胞:Mkrtichyan,M.etal.(2011)Anti-PD-1synergizeswithcyclophosphamidetoinducepotentanti-tumor. EuropeanJournalofImmunology.Epubaheadofprint.
从肺癌组织中分选髓系衍生抑制细胞(myeloid-derivedsuppressorcells):Shina,P.etal.(2011)Myeloid-derivedsuppressorcellsexpressthedeathreceptorFasandapoptoseinresponsetoTcell–expressedFasL.Blood117:5381–5390.
从肝肿瘤中分选CD4+T细胞:Bouabe,H.etal.(2011)NovelHighlySensitiveIL-10–β-LactamaseReporterMouseRevealsCellsoftheInnateImmuneSystemasSubstantialSourceofIL-10InVivo.
JournalofImmunology.Epubaheadofprint.
从肾脏肿瘤组织中提取蛋白质:Weiss,J.etal.(2010)Macrophage-dependentnitricoxideexpressionregulatestumorcelldetachmentandmetastasisafterIL-2/anti-CD40immunotherapy.JournalofExperimentalMedicine207:2455–2467.
从乳腺肿瘤中提取蛋白质:Montero,J.etal.(2011)P-Rex1participatesinNeuregulin-ErbBsignaltransductionanditsexpressioncorrelateswithpatientoutcomeinbreastcancer.Oncogene30:
1059–1071*.
阴道组织分选CD4+T细胞和巨噬细胞:Wheeler,L.etal.(2011)InhibitionofHIVtransmissioninhumancervicovaginalexplantsandhumanizedmiceusingCD4aptamer-siRNAchimeras.JournalofClinicalInvestigation121:2401–2412.
大鼠样品脑提RNA:Bonneh-Barkay,D.etal.(2010YKL-40ExpressioninTraumaticBrainInjury:AnInitialAnalysis.JournalofNeurotrauma27:1215–1223.
肺检测结核分枝杆菌细菌载量:Foo,G.etal.(2011)Tcellmonitoringofchemotherapyinexperimentalrattuberculosis.AntimicrobialAgentsandChemotherapy55:3677–3683.
脾脏分选单核细胞:Gomez,A.etal.(2011)ProteasomeInhibitionwithBortezomibDepletesPlasmaCellsandAutoantibodiesinExperimentalAutoimmuneMyastheniaGravis.Journalof
Immunology186:2503–2513.
检测脾脏匀浆中阿霉素:Kaminskas,L.etal.(2011)Acomparisonofchangestodoxorubicinpharmacokinetics,antitumoractivity,andtoxicitymediatedbyPEGylateddendrimerandPEGylatedliposomedrugdeliverysystems.Nanomedicine:Nanotechnology,Biology,andMedicine.Epubaheadofprint.
胸腺分选单核细胞:Gomez,A.etal.(2011)ProteasomeInhibitionwithBortezomibDepletesPlasmaCellsandAutoantibodiesinExperimentalAutoimmuneMyastheniaGravis.JournalofImmunology186:2503–2513.
气管RNA提取:Remund,K.etal.(2009)TheMacrolideClarithromycinInhibitsExperimentalPost-transplantBronchiolitisObliterans.ExperimentalLungResearch35:830–840.
其他样品骆驼肝脏RNA提取:Elrobh,M.etal.(2011)MolecularCloningandCharacterizationof
CDNAEncodingaPutativeStress-InducedHeat-ShockProteinfromCamelusdromedarius.InternationalJournalofMolecularScience12:4214–4236.
骡马肝脏RNA提取:Matthiesen,C.etal.(2010)Feedingmink(Neovisonvison)aprotein-restricteddietduringpregnancyinduceshigherbirthweightandalteredhepaticgeneexpressionintheF2offspring.BritishJournalofNutrition104:544–553*.
仓鼠肺分选淋巴细胞:Hammerbeck,C.etal.(2011)CellsAreNotRequiredforPathogenesisintheSyrianHamsterModelofHantavirusPulmonarySyndrome.JournalofVirology.Epubahead
ofprint.
仓鼠脾脏分选淋巴细胞:Hammerbeck,C.etal.(2011)CellsAreNotRequiredforPathogenesisintheSyrianHamsterModelofHantavirusPulmonarySyndrome.JournalofVirology.Epubahead ofprint.
线虫RNA提取:Gleason,J.etal.(2010)Wntsignalingcontrolsthestemcell-likeasymmetricdivisionoftheepithelialseamcellsduringC.eleganslarvaldevelopment.DevelopmentalBiology348:58–66
蜱DNA提取:Dobec,M.etal.(2009)RickettsiahelveticainDermacentorreticulatusTicks.EmergingInfectiousDiseases15:98–100*.
番茄从番茄组织匀浆中提取尼古丁:Yasinok,A.etal.(2009)Graftingtomatoplantontobaccoplantanditseffectontomatoplantyieldandnicotinecontent.JournaloftheScienceofFoodandAgriculture89:1122–1128*.
提取DNA:Reid,A.etal.(2011)ConstructionofanintegratedmicrosatelliteandkeymorphologicalcharacteristicdatabaseofpotatovarietiesontheEUcommoncatalogue.EUPHYTICA;DOI10.1007/s10681-011-0462-6*.
黄蜂提取RNA:Wetterwald,C.etal.(2010)Identificationofbracovirusparticleproteinsandanalysisoftheirtranscriptlevelsatthestageofvirionformation.JournalofGeneralVirology91:2610–2619.