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Baculovirus Expression Products
From The Experts
| Baculovirus expression vectors |
| The simplest and the quickest baculovirus protein expression vectors. Genetically optimized for producing 100% recombinant virus in a single step by eliminating the need for plaque purification. |
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| The original flashBAC vector is still a great choice for most projects where the gene to be expressed is likely to be simple and expressed in the cytoplasm or nucleus. | |
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| flashBACGOLD is enhanced by the deletion of the chitinase and cathepsin protease genes. It provides superior levels of expression for any protein that is destined for secretion or to be inserted in the membrane, or for any protein that might be particularly liable to degradation. | |
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| The latest development of the flashBAC technology, flashBACULTRA, contains deletions of the chitinase and cathepsin genes, as above, but also deletions of the p10, p74 and p26 genes and provides the best choice for the most difficult to express proteins. Suitable for cytoplasmic, nucleus, secreted and membrane proteins. | |
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| A combination of flashBAC, flashBACGOLD and flashBACULTRA in a single kit. 3 reactions of each allows you to select the best expression vector for your protein. |
| Baculovirus protein expression kits |
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 | The perfect choice for scientists wanting to upgrade to the flashBAC baculovirus expression platform. An ALL-IN-ONE kit contains all the components needed to get started with recombinant protein expression in insect cells. Kit contains flashBAC ULTRA baculovirus, baculoFECTIN transfection reagent, control transfer plasmid, baculoGROW insect cell media and Sf9 insect cells. The components in the kit have been optimised to complement each other and to produce best titres of recombinant virus and yields of proteins to help ensure your success. |
| Baculovirus titration |
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| A Q-PCR based one-step virus titration kit which can give an accurate result within 2 hours without the need for plaque assays or immunoassays. | |
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 | Is a novel ALL-IN-ONE virus extraction and titration kit. Both baculovirus DNA extraction and Q-PCR based quantification have been combined into one convenient kit. Virus DNA extraction requires no spin columns and is carried out in a single step using a thermocycler. The primers, probe, mastermix and controls for Q-PCR are all included in the kit. |
| Baculovirus expression and titration |
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| The convenience and quality of both baculoQUANT and any variant of the flashBAC range combined into one great value kit!. |
| Transfer plasmids |
| A selection of transfer plasmids that allow simple cloning and facilitate homologous recombination into the flashBAC system. These can offer further improvements to protein expression and modification. |
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| Designed for high level expression of foreign genes under the powerful AcMNPV polyhedrin (polh) promoter. Contain a Col E1 origin of replication and an Ampicillin resistance gene for selection in E. coli. | |
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| These vectors greatly ease the purification of the recombinant proteins since the 6×His-containing fusion proteins bind with high affinity to Ni-NTA Agarose. The vectors are available with a choice of either N or C terminal His tags. |
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| Protein expression is driven by the AcMNPV p6.9 promoter which provides earlier expression than the polh promoter used in pOET1 and pOET2. Suitable when expressing proteins that require extensive post translational modifications. | |
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| Is a dual promoter baculovirus transfer vector designed for high level expression of two foreign genes simultaneously under the powerful AcMNPV polh promoter and the very late p10 promoter. This vector is also suitable for the expression of multi-subunit proteins. |
| Transfection Reagents |
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| | This novel nanoparticle based transfection reagent has been especially developed and optimised for the transfection of insect cells. With its unique characteristics it yields greater levels of transfection. |
| Insect cells |
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| | The Sf9 cells are adapted to serum-free suspension culture in baculoGROW media. The cells can be used for transient or stable expression of recombinant proteins, as a monolayer for transfection and production of recombinant baculovirus or for the propagation of baculovirus stocks. |
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| Are Sf9 cells that have been engineered to stably express an additional protein. The presence of this protein leads to prolonged longevity and increased recombinant protein production in baculovirus infected SuperSf9 cells compared to standard Sf9 cells. There are 3 SuperSf9 cell lines, each capable of enhancing the expression of different types of recombinant proteins. |
| Insect cell media |
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| | Is a serum- and protein-free medium. The medium has been specially formulated to facilitate the growth of Spodoptera frugiperda cell lines (such as Sf9 and Sf21), Drosophila S2 cells and other cells such as Trichoplusia ni (T.ni or Hi-5) cells. baculoGROW can be used for culturing adherent cells and suspension cultures. |
| Transfer plasmid sequencing primers |
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| | The sequencing primers are used to sequence inserts cloned into the multiple cloning site of any of the pOET transfer vectors |