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BaculovirusExpressionProducts
FromTheExperts
| Baculovirusexpressionvectors |
| Thesimplestandthequickestbaculovirusproteinexpressionvectors.Geneticallyoptimizedforproducing100%recombinantvirusinasinglestepbyeliminatingtheneedforplaquepurification. |
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| TheoriginalflashBACvectorisstillagreatchoiceformostprojectswherethegenetobeexpressedislikelytobesimpleandexpressedinthecytoplasmornucleus. | |
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| flashBACGOLDisenhancedbythedeletionofthechitinaseandcathepsinproteasegenes.Itprovidessuperiorlevelsofexpressionforanyproteinthatisdestinedforsecretionortobeinsertedinthemembrane,orforanyproteinthatmightbeparticularlyliabletodegradation. | |
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| ThelatestdevelopmentoftheflashBACtechnology,flashBACULTRA,containsdeletionsofthechitinaseandcathepsingenes,asabove,butalsodeletionsofthep10,p74andp26genesandprovidesthebestchoiceforthemostdifficulttoexpressproteins.Suitableforcytoplasmic,nucleus,secretedandmembraneproteins. | |
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| AcombinationofflashBAC,flashBACGOLDandflashBACULTRAinasinglekit.3reactionsofeachallowsyoutoselectthebestexpressionvectorforyourprotein. |
| Baculovirusproteinexpressionkits |
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 | TheperfectchoiceforscientistswantingtoupgradetotheflashBACbaculovirusexpressionplatform.AnALL-IN-ONEkitcontainsallthecomponentsneededtogetstarted with recombinant protein expression in insect cells. Kit contains flashBAC ULTRAbaculovirus,baculoFECTINtransfectionreagent,controltransferplasmid,baculoGROWinsectcellmediaandSf9insectcells.Thecomponentsinthekithavebeenoptimisedtocomplementeachotherandtoproducebesttitresofrecombinantvirusandyieldsofproteinstohelpensureyoursuccess. |
| Baculovirustitration |
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| AQ-PCRbasedone-stepvirustitrationkitwhichcangiveanaccurateresultwithin2hourswithouttheneedforplaqueassaysorimmunoassays. | |
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 | IsanovelALL-IN-ONEvirusextractionandtitrationkit.BothbaculovirusDNAextractionandQ-PCRbasedquantificationhavebeencombinedintooneconvenientkit.VirusDNAextractionrequiresnospincolumnsandiscarriedoutinasinglestepusingaThermocycler.Theprimers,probe,mastermixandcontrolsforQ-PCRareallincludedinthekit. |
| Baculovirusexpressionandtitration |
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| TheconvenienceandqualityofbothbaculoQUANTandanyvariantoftheflashBACrangecombinedintoonegreatvaluekit!. |
| Transferplasmids |
| AselectionoftransferplasmidsthatallowsimplecloningandfacilitatehomologousrecombinationintotheflashBACsystem.Thesecanofferfurtherimprovementstoproteinexpressionandmodification. |
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| DesignedforhighlevelexpressionofforeigngenesunderthepowerfulAcMNPVpolyhedrin(polh)promoter.ContainaColE1originofreplicationandanAmpicillinresistancegeneforselectioninE.coli. | |
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| Thesevectorsgreatlyeasethepurificationoftherecombinantproteinssincethe6×His-containingfusionproteinsbindwithhighaffinitytoNi-NTAAgarose.ThevectorsareavailablewithachoiceofeitherNorCterminalHistags. |
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| ProteinexpressionisdrivenbytheAcMNPVp6.9promoterwhichprovidesearlierexpressionthanthepolhpromoterusedinpOET1andpOET2.Suitablewhenexpressingproteinsthatrequireextensiveposttranslationalmodifications. | |
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| IsadualpromoterbaculovirustransfervectordesignedforhighlevelexpressionoftwoforeigngenessimultaneouslyunderthepowerfulAcMNPVpolhpromoterandtheverylatep10promoter.Thisvectorisalsosuitablefortheexpressionofmulti-subunitproteins. |
| TransfectionReagents |
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| | Thisnovelnanoparticlebasedtransfectionreagenthasbeenespeciallydevelopedandoptimisedforthetransfectionofinsectcells.Withitsuniquecharacteristicsityieldsgreaterlevelsoftransfection. |
| Insectcells |
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| | TheSf9cellsareadaptedtoserum-freesUSPensioncultureinbaculoGROWmedia.Thecellscanbeusedfortransientorstableexpressionofrecombinantproteins,asamonolayerfortransfectionandproductionofrecombinantbaculovirusorforthepropagationofbaculovirusstocks. |
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| AreSf9cellsthathavebeenengineeredtostablyexpressanadditionalprotein.ThepresenceofthisproteinleadstoprolongedlongevityandincreasedrecombinantproteinproductioninbaculovirusinfectedSuperSf9cellscomparedtostandardSf9cells.Thereare3SuperSf9celllines,eachcapableofenhancingtheexpressionofdifferenttypesofrecombinantproteins. |
| Insectcellmedia |
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| | Isaserum-andprotein-freemedium.ThemediumhasbeenspeciallyformulatedtofacilitatethegrowthofSpodopterafrugiperdacelllines(suchasSf9andSf21),DrosophilaS2cellsandothercellssuchasTrichoplusiani(T.niorHi-5)cells.baculoGROWcanbeusedforculturingadherentcellsandsuspensioncultures. |
| Transferplasmidsequencingprimers |
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| | ThesequencingprimersareusedtosequenceinsertsclonedintothemultiplecloningsiteofanyofthepOETtransfervectors |