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| 货号： | B0114 |
| 保存条件： | 4oC. |
| 供应商： | 上海经科化学科技有限公司 |
| 保质期： | 10XTurboBiotinylationMixat4oC. |
| CAS号： | 无 |
| 英文名： | TurboBiotinylationKit |
| 数量： | 期货 |
| 规格： | 50XTurboBiotinylase(0.05mg) |
| Description: Accelagen"sTurboBiotinylationTM Kitisanenhancedbiotinylationkitforinvitrobiotinylationofbiotin-acceptingproteinsandrecombinantproteinswithAviTagTM orotherbiotin-acceptingpeptides.CombiningconcentratedTurboBiotinylaseandstable10XTurboBiotinylationMix,thiskitoffershighefficiencyinvitrobiotinylationfrom4-37 oC.ItdoesnotrequireanyspecialbufferforitsactivityandiscompatIBLewithawiderangeofbuffersthataremostsuitablefortargetproteins.ThiskitworksequallywellacrosspH5.5-10,between0-1MNaCl,andin10%glycerol. ProteinType: biotinligase Source: E.coli SpecificActivity: TurboBiotinylasehasaspecificactivityof>8,000Units/µg. OneunitofTurboBiotinylasecompletelybiotinylates1pmolofasubstrateprotein(23kDa)atconcentrationof1mg/mlwithin1hourat25 oC. StorageandHandling: TurboBiotinylationKitisshippedwithgelpacks. Thekitretainsitsfullactivityafterincubationat25 oCforoveraweek. Allcomponentsofthekitcanbestoredat4 oCforatleast6months.Forlongtermstorage,store50XTurboBiotinylaseat-80 oCand10XTurboBiotinylationMixat4 oC. |
 | TurboBiotinylaseisastableultra-purerecombinantE.coliBirAbiotinligasepurifiedfromE.coliandhasacalculatedmolecularweightof35.6kDa.Itisfreeofproteaseornucleasecontamination. |
 | Theefficiencyofbiotinylationcanbemeasuredbymassspectrometry.Biotinylationadds226.31Datoaprotein. Alternatively,biotinylationcanbeevaluatedbystreptavidin-agarosepull-down.ACoomassiestainedgelwillvisuallyshowtheefficiencyofbiotinylation.
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