| Description: TurboNucleaseTMisanultra-purerecombinantformofSerratiamarcescensextracellularendonuclease(encodedbythesamegeneasBenzonase)producedinE.coliusingaproprietaryprocess.Thisnon-specificendonucleasehydrolyzesbothsingle-anddouble-strandednucleicacids(DNAandRNA)to5’-phosphorylatedoligonucleotidesof1-4basesinlength.TurboNucleaseisahighlypurifiedhomodimerof27kDasubunitsthathasexceptionallyhighspecificactivityandisfreeofproteaseactivity.TurboNucleaseisidealtodigestnucleicacidsandtoreduceviscosityduringproteinpurificationandsamplepreparation. ProteinType: Endonuclease Source: E.coli UnitDefinition: OneunitofTurboNucleaseconverts1.0OD260ofsalmonspermDNAintoacid-solublenucleotidesin30minutesat37oCinareactionbufferof50mMTris-HCl,pH8.0and1mMMgCl2.Thiscorrespondstocompletedigestionof50µgofsalmonspermDNAintooligonucleotides.TurboNucleasehassimilarspecificactivityasBenzonase Storage: StoreTurboNucleaseat-20oC. EndotoxinLevel: < 0.1 EU / 1,000 units of TurboNuclease as determined by the Endo-Safe LAL Assay. |
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