题目不详
MOPS高温灭菌后颜色会变黄,但也可以使用。由于不知道会不会有什么不好的影响,所以就不推荐灭菌。RNA用的时候一般用处理好的水配置后过滤除菌。参考:1 sigmahttp://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/2/m1254pis.pdf MOPS Free Acid is ...... Sterilization should be done by filtrationthrough 0.2 µm filters. Autoclaving is not recommended for any sulfonic acid buffers. If buffers must benuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MOPSsolutions have been autoclaved, they turn yellow (although pH does not change measurably). The identityof the yellow breakdown product is unknown.2 《分子克隆》向左转|向右转