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| Catalognumber | HM1070 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Application | Flowcytometry,Frozensections,Immunofluorescence,Immunoprecipitation,Westernblot |
| ApplicationNotes | FC:AntibodyFA-11stainsboth-intraandextracellularmacrosialin.Forintracellularstaining-THP-1cellswerepermeabilizedwithbuffercontaining0.5%saponin.TheTHP-1cellsweretreatedanFcreceptorblockingsolutiontoblocknonspecificbinding.AsnegativecontrolanisotypematchedcontrolIgGwasused.(Ref.3) W:Anon-reducedsampletreatmentandSDS-Pagewasused.Thebandsizesiare75-120kDadependingonglycosylationpatternofmacrosialin.(Ref.5). IHC-F:-5 µm;Mtissuesectionswerefixedinacetonefor10minutesatroomtemperature.As-negativecontrolsprimaryantibodieswereomittedorreplacedbyratmAbstounrelatedantigens(Ref.2). |
| Use | Forimmunohistochemistry,flowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser’sexperimentalsetting. |
| Immunogen | PurifiedglycoproteinsfromP815cellline |
| Isotype | RatIgG2a |
| Species | Mouse |
| Alias | CD68antigengp110 |
| PositiveControl | murinemacrophage-likecelllineRAW264.7(Ref.5) |
| References | 1.Smith,Metal;Differentialexpressionofmurinemacrophagesurfaceglycoproteinantigensinintracellularmembranes.JCellSci1987,87:113 2.Rabinowitz,Setal;Macrosialin,amacrophage-restrictedsialoproteindifferentiallyglycosylatedinresponsetoinflammatorystimuli.JExpMed1991,174:827 3.Ramprasad,Metal;CellsurfaceexpressionofmousemacrosialinandhumanCD68andtheirroleasmacrophagereceptorsforoxidizedlowdensitylipoprotein.ProcNatlAcadSci1996,93:14833 4.DaSilva,Retal;Phagocytosisstimulatesalternativeglycolysationofmacrosialin(mouseCD68),amacrophage-specificendosomalprotein.BiochemJ1999,338:687 5.DeBeer,Metal;LackofadirectroleformacrosialininoxidizedLDLmetabolism.JLipidRes2003,44:674 6.Apostolopoulos,Jetal;Thecytoplasmicdomainoftissuefactorinmacrophagesaugmentscutaneousdelayed-typehypersensitivity.JLeukocBiol2008,83:90 7.Rickard,Aetal;DeletionofMineralocorticoidReceptorsFromMacrophagesProtectsAgainstDeoxycorticosterone/Salt-InducedCardiacFibrosisandIncreasedBloodPressure.Hypertension2009,54:537 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Tumorimmunology |