[白术内酯]关苍术HPLC指纹图谱研究及白术内酯Ⅰ和Ⅲ的含量分析

【作者】刁继红；杨绍群；刘爽；梁刚；梁贺；张善玉；

【Author】DIAOJi-hong(Dept.ofPharmacy,TheAffiliatedHospitalofYanbianUniversity,JilinYanji133000,China)YANGShao-qun,LIUShuang,LIANGGang,LIANGHe,ZHANGShan-yu(KeyLaboratoryforNaturalResourceofChangbaiMountain&FunctionalMolecules,MinistryofEducation,JilinYanji133000,China)

【机构】延边大学附属医院药剂科；长白山生物资源与功能分子教育部重点实验室；

【摘要】目的:建立关苍术的高效液相色谱(HPLC)指纹图谱,并测定关苍术中白术内酯Ⅰ和Ⅲ的含量。方法:指纹图谱和含量测定研究的色谱柱分别为KromasilC18(250mm×4.6mm,5μm)和ODSHypersilC18(250mm×4.6mm,5μm),检测波长分别为240nm和220nm,流动相分别为水-乙腈(梯度洗脱)和甲醇-水(60:40,V/V),流速为1mL.min-1,进样量为10μL。结果:关苍术指纹图谱中共标定了9个共有指纹峰,10批样品的相似度均>0.9。白术内酯Ⅰ和Ⅲ的进样量分别在50.1～417.5ng(r=0.9997)和49.5～412.5ng(r=0.9998)范围内与各自峰面积积分值呈良好线性关系,平均加样回收率分别为101.02%和97.12%,RSD分别为4.32%和4.01%(n均为9)。结论:所建指纹图谱具有较好的精密度、重复性和稳定性;所建含量测定方法简单、灵敏、结果可靠,均可用于关苍术的品质评价和质量控制。更多还原

【Abstract】OBJECTIVE:ToestablishHPLCfingerprintofAtractylodesjaponica,andtodeterminethecontentsofatractylenolideⅠandatractylenolideⅢinA.japonica.METHODS:FingerprintstudyandcontentdeterminationwereperformedonKromasilC18column(250mm×4.6mm,5μm)andODSHypersilC18column(250mm×4.6mm,5μm);thedetectionwavelengthwassetat240nmand220nm;mobilephaseconsistedofwater-acetonitrile(gradientelution)andmethanol-water(60:40,V/V)attheflowrateof1mL.min-1.Theinjectionvolumewas10μL.RESULTS:Therewere9commonpeaksinthefingerprintsofA.japonica,andthesimilarityof10batchesofsampleswashigherthan0.9.Thelinearrangeswere50.1～417.5ng(r=0.9997)foratractylenolideⅠand49.5～412.5ng(r=0.9998)foratractylenolideⅢ.Theaveragerecoverieswere101.02%and97.12%,andRSDswere4.32%and4.01%,respectively(n=9).CONCLUSION:Establishedfingerprinthasgoodprecision,reproducibilityandstability;themethodissimple,sensitiveandreliable.ItissuitableforqualityevaluationandcontrolofA.japonica.

【药理作用】白术内酯Ⅰ能促进胃肠道消化吸收功能,是白术键脾的有效成分之-.具有抗炎、抗肿瘤作用，该成分还具有调节胃肠道功能和促进营养物质吸收的功能。