Avalanche®CRISPR Transfection Reagent,,
CRISPR-Cas9hastriggeredarevolutioninwhichlaboratoriesaroundtheworldareusingthetechnologyforinnovativeapplicationsinbiology.Thistechnologyisabletosystematicallyanalyzegenefunctionsinmammaliancells,studygenomicrearrangementsandtheprogressionofcancersorotherdiseases,andpotentiallycorrectgeneticmutationsresponsibleforinheriteddisorders.
TheCRISPR-Cassystemisaprokaryoticimmunesystemthatconfersresistancetoforeigngeneticelementssuchasplasmidsandphages,andprovidesaformofacquiredimmunity.CRISPRspacersrecognizeandcuttheseexogenousgeneticelementsinamanneranalogoustoRNAinterferenceineukaryoticorganisms.CRISPRsarefoundinapproximately40%ofsequencedbacteriagenomesand90%ofsequencedarchaea.
TheCRISPRinterferencetechniquecanbeappliedineukaryoticcells.BydeliveringtheCas9proteinandappropriateguideRNAs(sgRNA)intoacell,theorganismsgenomecanbecutatanydesiredlocation.
TheCas9enzyme(green)generatesbreaksindouble-strandedDNAbyusingitstwocatalyticcenterstocleaveeachstrandofaDNAtargetsitenexttoaPAMsequenceandmatchingthe20-nucleotidesequenceofthesingleguideRNA(sgRNA).TheTransfectionReagent:OneofthemostimportantfactorsthatdeterminesasuccessfulCRISPR-Cas9mediatedgenometargetingistheefficiencyofdeliveringfunctionalCas9geneandsgRNAintothecells(thetransfectionprocess).EZBiosystems,thetransfectionandgeneexpressionexperts,hasspecificallydevelopedthetransfectionreagentforCRISPR-Cas9geneeditinginmammaliancells----Avalanche-CRISPRTransfectionReagent:
Features:
Greatforco-transfectionoflargeplasmidswitheithersmallplasmidsorsmallRNA,suchassgRNAGreatforCRISPR-Cas9geneeditingindifficult-to-transfectcells,suchasprimarycells,suspensionculturecells,orneuralcells.
Avalanche-CRISPRoutperformsotherbrandsoftransfectionreagentsontransferringCas9/GFPco-expressionplasmidsintosomedifficult-to-transfectcells.GFPexpressionindifferenttypeofcellsaftertransfectingpCas9-GFPplasmid(Addgene#44719)byusingAvalanche-CRISPR,Lipofectamine2000(L2K),andLipofectamine3000(L3K).Thecellstestedare:Humandermalfibroblast,BJAB(humanBurkittlymphomacell),HumaniPScell,NSC-34(MouseMotorNeuronHybridCell),C2C12cell(mousemyoblast),andHUVEC(HumanUmbilicalVeinEndothelialCell)
Orders,ifenteredpriorto4:00pmEasternStandardTime,maybeshippedthesameday(MondaythroughThursday)viaFedExStandard/PriorityOvernightforUSdomesticcustomers;thesameday(Friday,Monday,Tuesday)viaFedExInternationalPriorityforInternationalcustomers(deliverinabout2-4days).
Theitemwillbeshippedatambienttemperature.Itwillbegoodfor1yearafterdeliveryifkeptrefrigerated.
