LOXIMVI Cell Avalanche® Transfection Reagent (human melanoma cell),Will be provided with the purchase of the reagent.,

TissueType:Skin
Histology:Melanoticmelanoma;Malignantmelanoma;nonepithelial
Metastaticsite:lymphnode
p53Status:1
ECADMethylation:34
MDRFunction:12
Institution:NorskHydrosInstitute,Norway
Contributor:Fodstad,O
Reference;IntJCancer.41:442-449,1988
LOX-IMVIisoneofthecelllinesoftheNCI-60panelwhichrepresentsdifferentcancertypesandhasbeenwidelyutilizedfordrugscreeningandmoleculartargetidentification.

TheTransfectionReagent:
LOX-IMVICellAvalancheTransfectionReagentisanewclassofuniquechemicalformulationsspecificallyformulatedandoptimizedfortransfectingLOX-IMVIcells.TheproprietaryformulationoflipidsandpolymersensuresthehighestpossibletransfectionefficienciesandviabilitiesforLOX-IMVIcells.Fordetailsofthedevelopingprocessofthisreagent,pleasegoto:HowdidEZBiosystemsdevelopAvalancheCelltype/celllinespecifictransfectionreagentseries?

Features:
Muchlessreagentneededforeachtransfection:0.5mlisabletotransfectabout1000wellsof24-wellplateDeliversingleormultipleplasmidsFreeofanimal-derivedcomponentsEasy-to-useprotocol:JustmixnucleicacidswiththereagenttoformcomplexesandsimplyaddthemixturetocellsActiveinserum-containingmediumSuitableforReverseTransfectionCompatiblewithtransfectioninanyplateformatEconomical:HighefficiencymeanslessamountofnucleicacidreagentisneededDevelopedandmanufacturedbyEZBiosystems
FIG.1.Highthroughputtestoftransfectionefficiency(determinedasRLU/mg)onLOX-IMVIcellsaftertransfectionofluciferasereportergenebyusingour172proprietarytransfectionformulasandseveralmostpopularcommercialtransfectionreagents.Theyellowboxshowedtheresultsof4commercialtransfectionreagents.TheredlinesmarkedourcandidateformulaswiththehighesttransfectionefficiencyforLOX-IMVIcells.Thistestresultwasconfirmedwithrepeatexperiments.Theonethatshowedtheoptimalbalanceofpotent&lowcytotoxicityamongthosecandidateformulasafterflowcytometryanalysisonthepercentageof7AADpositivecellswaslaternamedasthisLOX-IMVICellAvalancheTransfectionReagent.
LOX-IMVICellAvalancheTransfectionReagent(humanmelanomacell)canalsobeusedonthefollowingcellswithhightransfectionefficiencies.
M14Cell
SK-MEL-2Cell
SK-MEL-5Cell
A-375Cell
SK-MEL-28Cell
MDCKCell
VeroCell
293Cell
293T/17CellRecommendedprotocolsforthesecellswillbeprovidedwiththereagent.Theprotocolsusuallyprovidesatisfactorytransfectionefficiencywithinvisiblecytotoxicity.However,optimizationmaybeneededforcertaintypeofcells.Optimizationsmayinclude:theamountofDNAandthistransfectionreagent;celldensity;transfectionreagent/DNAratio,orincubationtimeforthemixtureoftransfectionreagent/DNAetc.Forbesttransfectionresult,werecommendusingtherespectivecelltype/celllinespecificAvalanchetransfectionreagents.Thosereagentshavebeenoptimizedonbothrecipesandprotocols,andhavebeenprovedtohavethebesttransfectionresultsfortherespectivecelllinesorprimarycells.YoucaneasilyfindtherespectiveAvalanchetransfectionreagentsspecificforyourcellsbyusingthefiltersofourproductlist.
Orders,ifenteredpriorto4:00pmEasternStandardTime,maybeshippedthesameday(MondaythroughThursday)viaFedExStandard/PriorityOvernightforUSdomesticcustomers;thesameday(Friday,Monday,Tuesday)viaFedExInternationalPriorityforInternationalcustomers(deliverinabout2-4days).

Theitemwillbeshippedatambienttemperature.Itwillbegoodfor1yearafterdeliveryifkeptrefrigerated.