Recombinant Human βNerve Growth Factor/βNGF (Ser122Ala241, E.coli) (C060)

RecombinantHumanbeta-NerveGrowthFactorisproducedbyourE.coliexpressionsystemandthetargetgeneencodingSer122-Ala241isexpressed.

Lyophilized

Lyophilizedproteinshouldbestoredat< -20°C, though stable at room temperature for 3 weeks.Reconstitutedproteinsolutioncanbestoredat4-7°Cfor2-7days.
Aliquotsofreconstitutedsamplesarestableat< -20°C for 3 months.

Alwayscentrifugetubesbeforeopening.Donotmixbyvortexorpipetting.Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100μg/ml.DissolvethelyophilizedproteininddH2O.
Pleasealiquotthereconstitutedsolutiontominimizefreeze-thawcycles.

Humanβ-NerveGrowthFactor(β-NGF)wasinitiallyisolatedinthemousesuBMAndibulargland.Itiscomposedofthreenon-covalentlylinkedsubunitsα,β,andγ;itexhibitsalltheBIOLOGicalactivitiesascribedtoNGF.ItisstructurallyrelatedtoBDNF,NT-3andNT-4andbelongstothecysteine-knotfamilyofgrowthfactorsthatassumestabledimericstructures.Β-NGFisaneurotrophicfactorthatsignalsthroughitsreceptorβ-NGF,andplaysacrucialroleinthedevelopmentandpreservationofthesensoryandsympatheticnervoussystems.Β-NGFalsoactsasagrowthanddifferentiationfactorforBlymphocytesandenhancesB-cellsurvival.Theseresultssuggestthatβ-NGFisapleiotropiccytokine,whichinadditiontoitsneurotropicactivitiesmayhaveanimportantroleintheregulationoftheimmunesystem.Humanβ-NGFshares90%sequencesimilaritywithmouseproteinandshowscross-speciesreactivity.