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另备SIGMA原装蛋白酶K 货号:P6556规格:100MG
ProteinaseK,Tritirachiumalbum
Serineproteasethatexhibitsstrongproteolyticactivityonawidevarietyofdenaturedandnativeproteinsofhighmolecularweight.Becauseofitsrapidproteolyticinactivationofendogenousnucleases,itcanbeusedforisolationofmRNAandhighmolecularweightDNA.PreferentiallycleavesbondsnexttothecarbonylgroupofN-substitutedhydrophobic,aliphatic,andaromaticaminoacids.InhibitedbyDFP,Hg2+,andPMSF.Notinactivatedbymetalchelators,sulfhydrylreagents,TLCK,orTPCK.
Productinformation
Form Whitelyophilizedsolid
Formulation Lyophilizedfromcalciumacetate
Molarmass 28500
Specificactivity ≥40mAnsonU/mgprotein
Unitdefinition Oneunitisdefinedastheamountofenzymethatwillliberate1.0µmoltyrosinefromcaseinperminat37°C,pH7.5.
Activity ≥30mAnsonU/mgdryweight
Contaminants DNase:nodetectablenickingactivitywithpBR322,incubationfor6hat37°C;RNase:noribonucleaseactivitywithMS2RNAdetectedafterincubationfor6hat37°C
pHvalue optimum7.5-12.0
Solubility 50mMTris-HCl,2mMcalciumacetate,pH8.0
ECnumber 3.4.21.14
CASnumber 39450-01-6
Storeandshipinformation
Storage +2°Cto+8°C