Cholera Toxin from Vibrio cholerae 别名: Cholera enterotoxin, Cholergen CAS号: 9012-63-9 MDL number: MFCD00130776 包装 Package size based on protein content. Reconstitution When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, ａnd 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator. Unit Definition One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthine （IBMX）. Physical form Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide ａnd sodium EDTA Biochem/physiol Actions Toxin consisting of an A subunit （27 kDa） surrounded by five B subunits （approximately 12 kDa each）, which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein （Gαs）, reducing GTPase activity ａnd activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12. 相关类型 Bacteria, Bioactive Small Molecules, C-CH, Cell Biology, Cell Signaling ａnd Neuroscience, G Protein Function, G Proteins ａnd Cyclic Nucleotides, Reagents, Toxins ａnd Venoms Quality Level PREMIUM assay ~95% （SDS-PAGE） form lyophilized powder composition Protein, ~5% Lowry-TCA solubility H2O: soluble10 mg/mL storage temp. 2-8°C
