Cholera Toxin

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CholeraToxinfromVibriocholerae 
别名: Choleraenterotoxin,Cholergen
CAS号:9012-63-9
MDLnumber:MFCD00130776
包装
Packagesizebasedonproteincontent.
Reconstitution
Whenreconstitutedat1mg/mLinwater,solutionwillcontain0.05MTrisbuffersalts,pH7.5,0.2MNaCl,0.003MNaN3,ａnd0.001MsodiumEDTA.Storereconstitutedsolutionsintherefrigerator.
UnitDefinition
Oneunitincreasesadenylatecyclaseactivity6-fold,measuredas[3H]-cAMPpresent,usingapprox.70,000CHOcellsafterincubationfor90minat37°Cwith[3H]-adeninefollowedbya15minincubationwith1mM3-isobutyl-1-methylxanthine（IBMX）.
Physicalform
LyophilizedpowdercontainingTrisbuffersalts,sodiumchloride,sodiumazideａndsodiumEDTA
Biochem/physiolActions
ToxinconsistingofanAsubunit（27kDa）surroundedbyfiveBsubunits（approximately12kDaeach）,whichattachthetoxintogangliosideGM1onthecellsurface.TheAsubunitcatalyzesADP-ribosylationoftheα-subunitofthestimulatoryGprotein（Gαs）,reducingGTPaseactivityａndactivatingtheα-subunit.ThisactivationofGαsleadstoanincreaseintheactivityofadenylylcyclase,resultinginincreasedlevelsofcAMP.AlsoADP-ribosylatestransducinintheeyerodoutersegments,inactivatingitsGTPaseactivity.CholeratoxinhasalsobeenreportedtoADP-ribosylatetubulin.Showntobeapotentmucosalvaccineadjuvant,inducingThelpercelltype2responsesbyinhibitingtheproductionofinterleukin-12.
相关类型Bacteria,BioactiveSmallMolecules,C-CH,CellBIOLOGy,CellSignalingａndNeuroscience, 
GProteinFunction,GProteinsａndCyclicNucleotides,Reagents,ToxinsａndVenoms
QualityLevelPREMIUM 
assay~95%（SDS-PAGE） 
formlyophilizedpowder 
compositionProtein,~5%Lowry-TCA  
solubilityH2O:soluble10mg/mL 
storagetemp.2-8°C