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Skeletalmuscleisanimportantsiteofinsulin-stimulatedglucosedisposalandoftenthesiteofinsulinresistanceinobesity.Humanprimaryculturedskeletalmyoblastscandirectlyreflectapatientsmetabolicphenotype,becausemanyofthesignalingpathwaysaremaintainedintact.ZenBiooffershumanprimaryskeletalmyoblastsfromavarietyofdonors,includingobesedonorswithType2diabetes.
Humanskeletalmyoblastssatellitecellsareisolatedfromconsentedpatientswithgoodrepresentationfromalllevelsofadiposityandage.Skeletalmyoblastsareavailablefrombothmaleandfemaledonors.Pooledlotsofcellsareavailabletoprovidelargelotsforscreeningandtodecreaseissueswithpatienttopatientvariability.
Cellsareavailablecryopreserved(500,000cells/vial).
QUALITYCONTROL:Allcellshavebeentestedformyotubedifferentiation.CellshavetestednegativeforHIV-1,HIV-2,HTLC-1,HTLV-2,HepatitisB,HepatitisCandmycoplasma.HumanskeletalmyoblastaredifferentiatedusingSkeletalMuscleDifferentiationMedium(cat#SKM-D)foratleast6days.Myogenicdifferentiationwasverifiedbyimmunohistochemicalstainingusingantibodiestodesminandmyosin.Thedifferentiatedmyocytesalsorespondtoinsulinstimulatedglucoseuptake.
Speciallyformulatedmediaareavailableformaintenanceanddifferentiationofouradultskeletalmyoblasts.
