eBioscience Super Bright 436 染料档案

eBioscience 最新推出了应用于紫光激发的超高性能新型荧光染料-Super Bright系列。该系列荧光染料均已最大发射波长命名。这些染料经过优化尤其适用于流式染色。与传统类似染料相比，Super Bright 染料的非特异性结合更少。Super Bright系列染料兼容其他eBioscience的染料、缓冲液、固定剂和UltraComp eBeads 补偿微球。

激发光：405nm

发射光：436nm

可替代染料：eFluor 450, Brilliant™ Violet 421 (BV421)

检测滤片：450/50 bandpass

档案详细资料：

1. Super Bright 436 vs Brilliant Violet 421：；亮度与BV421相当甚至更好。

(A)\Human peripheral blood cells were stained with Anti-CD19 (clone HIB19)conjugated to Super Bright 436 (purple histogram) or to Brilliant Violet 421 (blue histogram) using the manufacturer’s recommended volume per test.

(B) and (C)\Mouse bone marrow cells were stained with Anti-CD117 (clone 2B8) APC and Anti-Ly-6A/E (clone D7) conjugated to either Super Bright 436 (B) or to Brilliant Violet 421

(C) at the same antibody concentration.

2. Super Bright 436 比eFluor 450亮度高

Mouse bone marrow cells were stained with Anti-CD117(clone 2B8) conjugated to (A) Super Bright 436, or (B) eFluor 450.

3. Super Bright 436光稳定性

Human peripheral blood cells were stained with Anti-CD19 (clone HIB19) conjugated to (A)Super Bright 436 or (B) to Brilliant Violet 421, andeither left in the dark (red histogram), or exposed to ambient light for four hours (orange histogram) or overnight (blue histogram).

4. Super Bright 436 固定后的稳定性

Stability studies indicate that Super Bright 436 exhibits a minimal loss of fluorescence when cells are exposed to various fixatives. Human peripheral blood cells were stained with Anti-CD27 (clone O323) Super Bright 436 and:

(A) were left unfixed (red histogram), or fixed in IC Fixation buffer for 30 minutes (blue histogram), overnight (orange histogram), or three days (green histogram), followed by a wash in Permeabilization buffer.

(B) were left unfixed (red histogram), or fixed in Foxp3/Transcription Factor buffer for 30 minutes (blue histogram), overnight (orange histogram), or three days (green histogram), followed by a wash in Permeabilization buffer.

(C) were left unfixed (red histogram), or fixed in IC Fixation buffer followed by 90% methanol for 30 minutes (blue histogram), overnight (orange histogram), or three days (green histogram), followed by a wash in Flow Cyometry Staining Buffer.

5. 兼容补偿微球

Mouse splenocytes were stained with a three-color panel comprised of Anti-CD45R/B220 (clone RA3-6B2) Super Bright 436, Anti-CD8a (clone 53-6.7) Super Bright 600, and Anti-CD4 (clone GK1.5) Super Bright 645. Compensation was set using (A) UltraComp eBeads microspheres (top row) or (B) with cells (bottom row). Compensation values with beads were similar to single color stained cells
(not shown).