eBioscience Super Bright 436 染料档案

eBioscience最新推出了应用于紫光激发的超高性能新型荧光染料-SuperBright系列。该系列荧光染料均已最大发射波长命名。这些染料经过优化尤其适用于流式染色。与传统类似染料相比，SuperBright染料的非特异性结合更少。SuperBright系列染料兼容其他eBioscience的染料、缓冲液、固定剂和UltraCompeBeads补偿微球。

激发光：405nm

发射光：436nm

可替代染料：eFluor450,Brilliant™Violet421(BV421)

检测滤片：450/50bandpass

档案详细资料：

1.SuperBright436vsBrilliantViolet421：；亮度与BV421相当甚至更好。

(A)HumanperipheralbloodcellswerestainedwithAnti-CD19(cloneHIB19)conjugatedtoSuperBright436(purplehistogram)ortoBrilliantViolet421(bluehistogram)usingthemanufacturer’srecommendedvolumepertest.

(B)and(C)MousebonemarrowcellswerestainedwithAnti-CD117(clone2B8)APCandAnti-Ly-6A/E(cloneD7)conjugatedtoeitherSuperBright436(B)ortoBrilliantViolet421

(C)atthesameantibodyconcentration.

2.SuperBright436比eFluor450亮度高

Mousebonemarrowcellswerestainedwith Anti-CD117(clone2B8) conjugatedto(A)SuperBright436,or(B)eFluor450.

3.SuperBright436光稳定性

Humanperipheralbloodcellswerestainedwith Anti-CD19(cloneHIB19) conjugatedto(A)SuperBright436or(B)toBrilliantViolet421,andeitherleftinthedark(redhistogram),orexposedtoambientlightforfourhours(orangehistogram)orovernight(bluehistogram).

4.SuperBright436固定后的稳定性

StABIlitystudiesindicatethatSuperBright436exhibitsaminimallossoffluorescencewhencellsareexposedtovariousfixatives.HumanperipheralbloodcellswerestainedwithAnti-CD27(cloneO323)SuperBright436and:

(A)wereleftunfixed(redhistogram),orfixedinICFixationbufferfor30minutes(bluehistogram),overnight(orangehistogram),orthreedays(greenhistogram),followedbyawashinPermeabilizationbuffer.

(B)wereleftunfixed(redhistogram),orfixedinFoxp3/TranscriptionFactorbufferfor30minutes(bluehistogram),overnight(orangehistogram),orthreedays(greenhistogram),followedbyawashinPermeabilizationbuffer.

(C)wereleftunfixed(redhistogram),orfixedinICFixationbufferfollowedby90%methanolfor30minutes(bluehistogram),overnight(orangehistogram),orthreedays(greenhistogram),followedbyawashinFlowCyometryStainingBuffer.

5.兼容补偿微球

Mousesplenocyteswerestainedwithathree-colorpanelcomprisedofAnti-CD45R/B220(cloneRA3-6B2)SuperBright436,Anti-CD8a(clone53-6.7)SuperBright600,andAnti-CD4(cloneGK1.5)SuperBright645.Compensationwassetusing(A)UltraCompeBeadsmicrospheres(toprow)or(B)withcells(bottomrow).Compensationvalueswithbeadsweresimilartosinglecolorstainedcells
(notshown).