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IsotypeControl

同型对照

同型对照(IsotypeControl),使用与一抗相同种属来源、相同亚型、相同剂量和相同的免疫球蛋白及亚型的免疫球蛋白,用于消除由于抗体非特异性结合到细胞表面而产生的背景染色。

同型对照

同型对照的主要目的是确定一抗的结合是特异性的,而不是非特异性的Fc受体或与其他蛋白的相互作用。还可以用来竞争性的结合抗体,与功能阻断抗体发挥同样的功能。

同型对照要与一抗的来源,Ig分型和标记完全一致。

如果一抗是多抗,可以用annormalserum(与一抗相同的正常血清)(mustbethesamespeciesasprimaryantibody)。Thiscontroliseasytoachieveandcanbeusedroutinelyinimmunohistochemicalstaining.这个可以咨询试剂商。同型对照为免疫荧光标记中的阴性对照。由于荧光标记单抗的组来源不同,应选用相同来源的未标记单抗作为同型对照来调整背景染色。举个例子:比如检测一抗为单抗的mouseantiratCD11b,cloneOX-42purifiedIgG,那么它的isotype是mouseIgG2a,所以可以用purified(纯化的)mouseIgG2a来做OX-42的同型对照(IsotypeControl)。一般的的生物技术公司和国内的代理都有出售。

同型对照:是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类,若使用直接免疫荧光染色法,同型对照也应标记荧光色素,如IgG1FITC、IgG2a、PE等。主要考虑了细胞的自发荧光、FC受体介导的抗体结合和非特异性抗体结合等影响因素。此外,同型对照与MoAb所标记的荧光色素、浓度、F:P比值(标记的荧光色素与免疫球蛋白分子的比值)应该相同为最佳,这对准确设定阴性与阳性细胞的界标有重要意义,切忌使用与MoAb不相匹配的同型对照,最好为同一实验室、采用相同工艺或方法制备(如同一品牌)的产品。


AbbexaisbasedattheWorldrenownedCambridgeSciencePark,inCambridge,UK.WeareasupplieranddistributorofBIOLOGicaltoolsforthelifescience,pharmaceuticaldevelopmentandbiotechnologysectors.Weprovidethescientificcommunitywithprimaryantibodies,secondaryantibodies,proteins,ELISAkitsandenzymesaswellasotherkitsandtoolsforuseinresearch.WorkingwithvariouslaboratoriesacrosstheWorld,weaimtodeveloprelevant,highquality,testedproductsforthebiomedicalresearchmarket.

Abbexaisadedicatedworldwideantibodysupplierandproteinsupplier.Whetheryouarecarryingoutacademicresearch,pharmaceuticalresearch,diseaseresearchorotherbiologicalresearch,ourmonoclonalantibodies,polyclonalantibodies,proteinsandbiologicalreagentsaredesignedtofacilitateyourresearch.Weappreciatethatsometimesitishardtofindtherightantibodytomatchyourneeds,andwethereforeofferacustomprotein,peptideandantibodyproductionpipeline.

Abbexaiscommittedtoprovidingscientistsandresearcherswiththebestcustomerexperienceandhighqualityproducts.Wewanttoprovideyouwiththebestsupportavailable,whichiswhyweguaranteecustomersatisfaction.Ifyouareunhappywiththeperformanceofyourproductwewillofferyouanimmediatereplacementorrefund.Pleasehavealookatourqualityguaranteeforfurtherdetails.


((2R,3R,4R)-3-(benzoyloxy)-4-fluoro-4-methyl-5-oxotetrahydrofuran-2-yl)methylbenzoate
abx181380 

CAS:874638-80-9


Purity: 0.9
US$362.50(250mg)
 ComparisonList
(+)alphaTocopherol
abx082409 
CAS:59-02-9
Conjugation: Unconjugated
US$145.00(5g)
 ComparisonList
(+)-AbscisicAcid
abx076833 
CAS:21293-29-8
Purity: 0.995
US$464.00(1mg)
 ComparisonList
(+)-Biotin-(PEO)4-amine
abx185673 
CAS:359860-27-8
Purity: >97%
US$841.00(0.5g)
 ComparisonList
(+)-Biotin-NHS;BIOTIN-NHS
abx182717 
CAS:35013-72-0
Purity: 0.98
US$246.50(1g)
 ComparisonList
(+)-Biotinhydrazide,Biotinhydrazide
abx187866 
CAS:66640-86-6
Purity: 0.95
US$232.00(100g)
 ComparisonList
(1-Ethoxycyclopropoxy)trimethylsilane
abx182720 
CAS:27374-25-0
Purity: 0.97
US$145.00(1g)
 ComparisonList
(1-Methyl-4-Piperidinyl)Methanamine
abx180083 
CAS:7149-42-0
Purity: 0.95
US$188.50(1g)
 ComparisonList
(1R,2R)-(-)-2-Benzyloxycyclohexylamine
abx184835 
CAS:216394-06-8
Purity: >95%
US$275.50(5g)
 ComparisonList
(1R,5S)-8-Benzyl-8-AzABIcyclo[3.2.1]Octan-3-One
abx187951 
CAS:28957-72-4
Purity: 0.95
US$623.50(100g)
 ComparisonList
Purity: 0.97
US$203.00(1g)
 ComparisonList
Purity: 0.97
US$261.00(1g)
 ComparisonList
(2-Amino-Pyridin-3-Yl)-Methanol
abx181398 
CAS:23612-57-9
Purity: 0.97
US$232.00(5g)
 ComparisonList
Purity: >95%
US$203.00(1g)
 ComparisonList
Purity: 0.95
US$333.50(1g)
 ComparisonList
(2-Hydroxypropyl)-Beta-Cyclodextrin
abx184506 
CAS:128446-35-5
Purity: 0.99
US$435.00(250g)
 ComparisonList
(2-Iodophenyl)methanol
abx184507 
CAS:5159-41-1
Purity: 0.99
US$174.00(25g)
 ComparisonList
Purity: >98%
US$348.00(250mg)
 ComparisonList
Purity: 0.95
US$319.00(250mg)
 ComparisonList
(3,4-Dimethylphenyl)hydrazine
abx182729 
CAS:60481-51-8
Purity: 0.98
US$145.00(5g)
 ComparisonList
(3-Chloro-2-Fluorophenyl)BoronicAcid
abx181413 
CAS:352535-82-1
Purity: 0.97
US$130.50(1g)
 ComparisonList
(3-Fluoro-4-Hydroxyphenyl)BoronicAcid
abx181415 
CAS:182344-14-5
Purity: 0.97
US$275.50(1g)
 ComparisonList
Purity: >96%
US$290.00(50g)
 ComparisonList
(4-Bromopyridin-2-yl)methanamine
abx181425 
CAS:865156-50-9
Purity: 0.97
US$406.00(1g)
 ComparisonList
Purity: 0.98
US$623.50(5g)
 ComparisonList
Purity: 0.977
US$580.00(1g)
 ComparisonList
(6-Methylpyridin-2-yl)methanamine
abx184861 
CAS:6627-60-7
Purity: >95%
US$261.00(1g)
 ComparisonList
(BMPS);3-MaleimidopropionicacidNHS
abx180108 
CAS:55750-62-4
Purity: 0.95
US$232.00(5g)
 ComparisonList
(DSS);Disuccinimidylsuberate
abx181439 
CAS:68528-80-3
Purity: 0.97
US$217.50(1g)
 ComparisonList
Purity: >97%
US$304.50(1g)
 ComparisonList
Purity: 0.96
US$188.50(1g)
 ComparisonList
Purity: >95%
US$174.00(1g)
 ComparisonList
(E)-3-(Furan-2-yl)acrylicacid
abx180109 
CAS:15690-24-1
Purity: 0.95
US$130.50(5g)
 ComparisonList
(E)-3-(Pyridin-3-Yl)AcrylicAcid
abx188019 
CAS:19337-97-4
Purity: 0.95
US$203.00(25g)
 ComparisonList
Purity: 0.99
US$1,203.50(5g)
 ComparisonList
(EMCA);6-Maleimidocaproicacid
abx182745 
CAS:55750-53-3
Purity: 0.98
US$159.50(5g)
 ComparisonList
(EMCS);6-MaleimidocaproicacidNHS
abx182746 
CAS:55750-63-5
Purity: 0.98
US$420.50(1g)
 ComparisonList
(GMBS);4-MaleimidobutyricacidNHS
abx182747 
CAS:80307-12-6
Purity: 0.98
US$304.50(1g)
 ComparisonList
Purity: 0.97
US$203.00(5g)
 ComparisonList
(PEO)4-amino-propionicacid
abx181441 
CAS:663921-15-1
Purity: 95%(1H-NMR).Productconformstostructureby 1H-NMR.
US$377.00(1g)
 ComparisonList
(PEO)4-mono-amine
abx180932 
CAS:86770-74-3
Purity: 95%(1H-NMR).Productconformstostructureby 1H-NMR.
US$246.50(1g)
 ComparisonList
(R)-(-)-Epichlorohydrin
abx182749 
CAS:51594-55-9
Purity: 0.98
US$159.50(25g)
 ComparisonList
(R)-2-(Tetrahydrofuran-3-Yl)Acetic-Acid
abx188023 
CAS:146255-25-6
Purity: 0.95
US$2,494.00(10g)
 ComparisonList
(R)-2-Aminobutan-1-ol
abx182758 
CAS:5856-63-3
Purity: 0.98
US$174.00(25g)
 ComparisonList
Purity: 0.96
US$217.50(1g)
 ComparisonList
(R,S)-NiguldipineHCl
abx076561 
CAS:119934-51-9
Purity: >98%
US$319.00(10mg)
 ComparisonList
Purity: 0.98
US$145.00(1g)


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