AntiMBL (human)

Anti-MBL (human)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
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HYB 131-102

Anti-MBL (human)

Mouse monoclonal antibody

HYB 131-10 is specific for MBL (mannan-binding lectin) from human serum or plasma.

MBL purified from human donor plasma.

ELISA, WB

Human

Not determined

IgG1/k

4153

1E2

The epitope is thought to be on the carbohydrate recognition domain and differs from that of HYB 131-01 and HYB 131-11.

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Protein-A purified

Liquid

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

4-8ºC without exposure to light. No precautions necessary during handling.

Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars (1). It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum.
Application 1: ELISA
HYB 131-10 reacts strongly with MBL. Strong reaction is seen in ELISA with MBL coated directly onto the microtiter well. In Western blotting HYB 131-10 reacts with human MBL in both its oligomerized state and as single protein chain of 26 kDa. HYB 131-10 can be used to measure MBL in plasma or serum but is not specific for oligomerized MBL when used in combination with itself or other MBL antibodies(1).
Application 2: WB
HYB 131-10 can be used in Western blotting.

温馨提示：不可用于临床治疗。