蚂蚁淘成为mRNAIn® Neuro Transfection Reagent授权代理商

mRNA-In®NeuroisanewmRNAtransfectionreagentspecificallydesignedandoptimizedforhighefficiencydeliverywithexceptionallylowcytotoxicityinneurons.Unlikeothercompetitorreagents,mRNA-In®NeurorequiresverylowamountsofRNAtoachievemaximumdeliverywhilemaintainingoptimalcellviABIlity.

mRNA-In®NeuroTransfectionReagentisanextgenerationlow-toxicitymRNAdeliverysystemforachievingmaximumtransfectionefficiencyinhard-to-transfectneurons.ItiswellsuitedforvariousneuroBIOLOGyapplications,includingneurotoxicityandneuriteoutgrowthdevelopment.DatahereshowsmRNA-In®typicallyachieves70%orgreatertransfectionefficiencyinprimaryneurons.WithmRNA-InNeuro®,mRNAexpressionlevelsusingcanbeadjustedbychangingtheamountofmRNAtransfection.Moreover,mRNA-In®-NeurorequiresverylowamountsofmRNA(upto5-foldlessthatcompetitorreagents)toachievemaximumtransfectionefficiencyinneurons,significantlyminimizingunintendedoff-targetimmuneactivationandtoxicityeffects.

LowAmountsof mRNA AchieveMaximumRNADeliveryinNeurons

Figure1.SuperiormRNATransfectionefficiencyinPrimaryNeurons –Primaryratcorticalneuronsculturedin24-wellplateswere transfected with mRNA-In® Neuro Transfection Reagentandincubatedovernightincompleteculturemedia.Maximumtransfectionefficiencywasmaintainedwithreducingamountsof mRNA,from 500ng(high)to 125ng (low)amountsof mRNA.Theaboveimagesweretaken48-hours post-transfection. IncontrasttoDNAtransfection,neuronstransfected withmRNA-In® Neuroshoweduniform GFP expressionfromhigh(500ng)toverylow(125ng)amountsof mRNA.

FeatureBenefits

• Maximumefficiencywithlowamountsof mRNA -Maximallyeffectivewithverylowamountsof mRNA,significantlyminimizingpotentialadverseoff-target cytotoxic effectsand supportingmaximumcellhealthandviability.

• Highexpression,lowtoxicity -Achieveshigh mRNA efficiencyandproteinexpression inhardto transfect neuronswhilemaintainingoptimalcellhealthfor uncompromised post-transfectionassay results.

• Versatileperformance -HighlyefficientandreproducIBLe transfectionperformanceacrossa widerangeofprimarycells.

• Rapidexpressionandeasytouse -Usewithouttheriskofintegrationwiththecellularhostgenomeandobservemorerapidexpressionasearlyas4hourspost-transfection. 

• Wellsuitedforvariousapplications -Showntobeeffectiveacrossarangeofapplications,including neurotoxicity and neurite development. 

  mRNA-In® NeuroShowsSuperiorPerformanceoverCompetitor 

  

  Figure2.  mRNA-In® NeuroOutperformsCompetitorReagents- mRNA-In NeuroTransfectionReagentwas comparedto MessengerMax™ fortransfectingneurons. Primarycorticalneuronsinculturefor6daysweretransfected with 500ng and 250ng amountsofmodified mRNA and2µlmRNA-In® Neuroor MessengerMax™ in24-wellplates.Cellswereincubatedovernightincompleteculturemediaandobserved 24hr and48hrs (notshown)post-transfection.Aboveimagesshowsignificantly highertransfectionefficiencyand GFP expressioninhealthyneuronsposttransfection.