IL-5 specific spots produced by 2x10E5 human PBMC. Stimulation: PMA/ionomycin.
Plate characteristics:
Transparent
Contents:
Coating antibodies
Biotinylated detector antibodies
Phi-labeled goat anti-biotin antibodies
Activator solutions
Blocking stock solution
Dilution buffer T
Tween-20
Transparent ELISPOT plates with lids and cover slips
Detailed manual
References list:
Immunological efficacy of heat shock protein 60 peptide DiaPep277 therapy in clinical type I diabetes.Huurman, V A L, et al.Clin. Exp. Immunol. 2008 Jun;152: 488-97
Development of type 1 diabetes despite severe hereditary B-lymphocyte deficiency.Martin, S, et al.N. Engl. J. Med. 2001 Oct 4;345: 1036-40
Association of T-cell reactivity with beta-cell function in recent onset type 1 diabetes patients.Pfleger, Christian, et al.J. Autoimmun. 2010 Mar;34: 127-35
Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop.Schloot, Nanette C, et al.J. Autoimmun. 2003 Dec;21: 365-76
