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B-27®添加剂是无血清添加剂，用于海马神经元和其他中枢神经系统（CNS）神经元的生长和保持其短期或长期活性。B-27®添加剂是50X的液体，可以与Neurobasal®培养基或Neurobasal®-A培养基组合使用，用于神经细胞培养而不需要再添加饲养层细胞。
B-27®添加剂适和应用于大多数神经细胞培养。
质量控制检测
B-27®添加剂进行了18天妊娠的原代大鼠（SpragueDawley）的胚胎海马神经元生长检测。通过了细菌和真菌的检测.B-27®也做了1X浓度的内毒素水平测试。
仅供研究使用。除非另有说明，不得用于任何动物或人类治疗或诊断。
CellType:
NeuronalCells
ProductSize:
10ml
Classification:
Serum-Free
OtherAdditives:
VitaminA,
AntioxidantCocktail,
Insulin
PurityorQualityGrade:
CellCultureGrade
GreenFeatures:
Sustainablepackaging
ShippingCondition:
DryIce
RegulatoryStatement:
ForResearchUseOnly.Notforuseindiagnosticprocedures.
引文和参考文献 （17）
•D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.
作者Congar P; Bergevin A; Trudeau LE杂志J Neurophysiol （2002） 87:1046-1102产品用途Using a culture system in which isolated DAergic neurons establish self-innervating synapses （"autapses"） that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents （EPSCs）. ID:PN64425
•Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.
作者Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;杂志Neuron （2002） 33:789-804产品用途Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator. ID:PN47418
•Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells.
作者Kabos Peter; Kabosova Andrea; Neuman Toomas;杂志J Biol Chem （2002） 277:8763-8769产品用途Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized （0.02 mg/ml trypsin in versene （Invitrogen） at 37 °C for 10 min. Cell sUSPension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement （Invitrogen）. Cells were washed with PBS （Invitrogen） ID:PN63471
•Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.
作者Lesuisse Christian; Martin Lee J;杂志J Neurobiol （2002） 51:9-23产品用途A long-term cell culture system was used to study maturation, aging, and death of cortical neurons. ID:PN60461
•An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.
作者Joseph-Bravo Patricia; PÃ
rez-MartÃnez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;杂志Brain Res Brain Res Protoc （2002） 9:93-104产品用途Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties ID:PN62028