Gibco/RPMI 1640 Medium, powder, HEPES/10 x 1 L/23400021

Description

RoswellParkMemorialInstitute(RPMI)1640mediumwasoriginallydevelopedtoculturehumanleukemiccellsinsUSPensionandasamonolayer.RPMI1640hassincebeenfoundsuitableforavarietyofmammaliancellsincludingHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytesandcarcinomas.LifeTechnologiesoffersavarietyofGibco®RPMI1640modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
Thecompleteformulationisavailable.

Gibco®RPMI1640isuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640containsbiotin,vitaminB₁₂andPABAwhicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.

ProductIntendedUse
Forinvitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.CustomersusingGibco®RPMI1640inamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromLifeTechnologiestoreferenceourTypeIIDrugMasterFile(DMF).

cGMPManufacturingandQualitySystem
Gibco®RPMI1640ismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,LifeTechnologiesoffersacomparableGibco®RPMI1640productmadeinourScotlandfacility(13018-015).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.

RPMI1640containsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640requiressupplementation,commonlywith10%FetalBovineSerum(FBS).RPMI1640usesasodiumbicarbonatebuffersystem(2.0g/L)andthereforerequiresa5-10%CO₂environmenttomaintainphysiologicalpH.

PowderformsofGibco®cellculturemediumrequiresodiumbicarbonatesupplementation,pHadjustment,andfiltrationatthetimeofpreparation(seeprotocolfordetails).