能不能帮我翻译下这段话啊

nThe extraction of RNA was performed using the RNeasy mini kit n(Qiagen). RT-qPCRs for RNA expression were performed by using nthe KAPA SYBR FAST One-step RT-qPCR kit (KAPA biosystems). nRT-qPCRs were performed on anMx3005p Real Time PCR System n(Stratagene). All PCR products were verified on agarose gels nstained with ethidium bromide. We designed and synthesized nthe following primers: ACE1-sense, 5′-caacatcaccacagagaccagcaa- n3′; ACE1-antisense, 5′-ggtgtctcgtacatagacctccac-3′; reninsense, n5′-caatccgagaaagcctgaag-3′; renin-antisense, 5′-ctgtgtcaccgtgattccac- n3′; GAPDH-sense, 5′-cgagatccctccaaaatcaa-3′; and nGAPDH-antisense, 5′-gtcttctgggtggcagtgat-3′

RNA的提取是用Qiagen公司生产的RNeasy 迷你试剂盒。

nThe萃取的RNA都使用了RNeasy迷你工具包n(试剂盒)。为进行RT-qPCRs RNA表达通过使用nthe KAPA SYBR快一步法rt定量酵素聚合反应技术装备(KAPA生物系统公司)。nRT-qPCRs anMx3005p进行实时PCR系统n(Stratagene)。所有产品都是在琼脂糖PCR验证凝胶nstained与溴化乙锭。我们设计并合成nthe以下底漆:5′,-caacatcaccacagagaccagcaa傻乎乎意义——n3′;5′傻乎乎反义,-ggtgtctcgtacatagacctccac-3;reninsense′,它们-caatccgagaaagcctgaag-3′′;肾素反义,5′- GAPDH-sense′-ctgtgtcaccgtgattccac n3;5′,-cgagatccctccaaaatcaa-3;nGAPDH-antisense′,5′-gtcttctgggtggcagtgat-3′

是这样的吧？ nThe萃取的RNA都使用了RNeasy迷你工具包n(试剂盒)。为进行RT-qPCRs RNA表达通过使用nthe KAPA SYBR快一步法rt定量酵素聚合反应技术装备(KAPA生物系统公司)。nRT-qPCRs anMx3005p进行实时PCR系统n(Stratagene)。所有产品都是在琼脂糖PCR验证凝胶nstained与溴化乙锭。我们设计并合成nthe以下底漆:5′,-caacatcaccacagagaccagcaa傻乎乎意义——n3′;5′傻乎乎反义,-ggtgtctcgtacatagacctccac-3;reninsense′,它们-caatccgag′