ESGRO® (LIF) ESG1106

ESGRO®(LIF)

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ESG1106	ESGRO®(LIF)[MurineLIF]	10⁶Units	2806
ESG1107	ESGRO®(LIF)[MurineLIF]	10⁷Units	12765

Description:ESGRO®(LIF)

TradeName:Chemicon(Millipore)

KeyApplications:StemCellCulture

ApplicationNotes:ApplicationsforESGRO®includeuseasareagentfortheinvitromaintenanceofthepluripotentialphenotypeofmurineEScells.

SUGGESTEDPROTOCOLS:ESCells:InD3andMBL-1pluripotentialEScellculturesitisroutinelyfoundthat1000UofESGRO®per1.0mLoftissueculturemediaisrequiredtomaintainEScellswithastemcellphenotype.SimilarconcentrationsofmLIFhavealsobeenusedforgermlinetransmissionofgeneticallyalteredEScells(E3).

Attherecommendedconcentration10⁷unitsofESGRO®issufficientfor10.0Loftissueculturemediaand10⁶unitsofESGRO®issufficientfor1.0Loftissueculturemedia.

Visitwww.esgro-lif.comforadditionalinformation

SpeciesReactivity:Mouse

UsageStatement:Unlessotherwisestatedinourcatalogorothercompanydocumentationaccompanyingtheproduct(s),ourproductsareintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.

Species:

Mouse

CellCultureMediaorReagentType:

ESGROSupplement

StemCellType:

MouseEmbryonicStemCells
InducedPluripotentStemCells

StemCellWorkflowStage:Maintenance&Expansion

MediaType:CellCultureMediaAdditives

Format:Purified

Presentation:ESGRO®issuppliedinliquidformas10⁶UnitsESGRO®in1.0mLofphosphatebufferedsalinewith1%w/vbovineserumalbuminBSAasacarrierforstABIlity.

StorageConditions:ESGRO®isshippedatambienttemperature.Acoldpackmaybeincludedforcustomerrelations,howeveritistrulynotneeded.ExtensivestabilitytestshavebeenperformedwithESGRO®duringdevelopment.Thisproductisstableforatleast18monthsfromthedateofmanufacture,intheconcentratedformordilutedinsteriletissueculturemedia,withnolossofactivityonEScells.ForlongtermstorageitisrecommendedthatESGROconcentratebestoredat4°C.Freeze-thawingwillreducepotency.Itisrecommendedthatpriortouse,ESGRO™shouldbedilutedinsteriletissueculturemediaandaliquotedtoaconvenientconcentration,thenstoredat4°C.Freeze-thawingshouldbeavoided.ESGRO™isstableforaminimumof7daysat37°C,5%CO2incubatorduringthecultureofEScells.

WARRANTY

Thehigheststandardsofqualitycontrolareusedinthemanufactureofthisproduct.Nowarrantyisprovidedthatthesaleoruseoftheproducteitheralone,incombinationwithotherproducts,orintheoperationofanyprocess,willnotinfringepatent,intellectualpropertyoranyotherrightsofthirdparties.Thisproductissuppliedforresearchpurposesonly.Itisnotforhumantherapeuticand/ordiagnosticuse.NOTE:ESGRO®shouldnotbeallowedtocomeincontactwithruminantanimalsorswine.

ManufacturedbyCHEMICONInternational,Inc.LIFisprotectedunderUSPatentnos.5,187,077,5,427,925,5,443,825,5,750,654and6,261,548,EuropeanPatentno.0285448andrelatedforeignpatentsandisnotavailableforresale.UseofrecombinantLIF,orCHEMICONsproprietaryESGRO®formulation,forthemaintenanceofembryonicstemcellsisprotectedworld-wideunderthefollowingpatents:US5,166,065,EP380646,andothers.

SpecificActivity:ESGRO®isassessedbothonES{embryonicstem}cells(D3&MBL-1)andonmurineM1myeloidleukemiccells(4).Astandardof50UnitsisdefinedastheconcentrationofESGRO®in1.0mLoftissueculturemediumthatinducesthedifferentiationof50%ofM1colonies(4).10⁶unitsisequivalenttoapproximately10μgofpureprotein,andissufficienttotreat1LofEScellculturemedia.

EmbryonicStemCellAssay:Differentiationinhibitionat1000units/mLMurinemyeloidleukemic,M1Assay:SpecificActivity>/=10⁸units/mg

Packaging:1mL

CellCultureReagentType:

Cytokines&GrowthFactors
CellCultureSupplements

GeneSymbol:

LIF
Emfilermin
CDF
MLPLI
HILDA
D-FACTOR

ProteinorEnzymeType:Cytokines&GrowthFactors

AlternateNames:MurineLIF

Concentration:1millionunits/1mL

Purity:TheactivecomponentmLIFhasbeenshowntobe>95%purebySDS-PAGE.ESGRO®supplied0.22micronsterilefiltered,andtestednegativeformycoplasmas.

ProductResources

Brochures
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Brochures
- CellutionsNewsletter:2008,Volume3
- CellutionsNewsletter:2008,Volume2
- CellutionsNewsletter:2008,Volume1
- CellutionsNewsletter:2007,Volume1
- MurineEmbryonicStemCellCulture
CertificatesofAnalysis
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CertificatesofAnalysis
- ESGRO®Supplement(106Units)-7240-102E
- MURINEESGRO106-ME9DN1177
DataSheets
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DataSheets
- ESGRO(LIF)-DataSheet
FAQs
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FAQs
Whyaremymouseembryonicstemcellsdifferentiatingandwhatpreventativemeasuresdoyourecommend?»Answer

Recommendation:RegularuseofPenicillin/Streptomycininmediacanoftenmaskalowlevelcontaminationwithagentssuchasmycoplasma.Itisrecommendedtohaveyourcelllinestestedformycoplasmaonaperiodicbasis.

Whyaremymouseembryonicstemcellsdifferentiatingandwhatpreventativemeasuresdoyourecommend?»Answer

Recommendation:RegularuseofPenicillin/Streptomycininmediacanoftenmaskalowlevelcontaminationwithagentssuchasmycoplasma.Itisrecommendedtohaveyourcelllinestestedformycoplasmaonaperiodicbasis.

Whyaremymouseembryonicstemcellsdifferentiatingandwhatpreventativemeasuresdoyourecommend?»Answer

Recommendation:RegularuseofPenicillin/Streptomycininmediacanoftenmaskalowlevelcontaminationwithagentssuchasmycoplasma.Itisrecommendedtohaveyourcelllinestestedformycoplasmaonaperiodicbasis.

Causeforthelackofchimeras:Timetakenpriortomicroinjection»Answer

Recommendation:ItisrecommendthatEScellsbemicroinjectedassoonaspossIBLeafterremovingthemfromthetissuecultureplates,andnotleftoniceorroomtemperatureforextendedperiods.

Causeforthelackofchimeras:EScellpassagenumber»Answer

Recommendation:Ingeneral;thebestchimerasaregeneratedfromlowpassagenumberEScells.Ifcelllineshavebeenculturedforahighnumberofpassages,itisrecommendedtogobacktoalowerpassagefrozenstock.

CauseofDifferation:Gelatin»Answer

Recommendation:Itispreferabletousecellculturegradegelatinatalltimes(evenifusingfeederlayers)asthegelatinminimisessurfacedifferencesonthetissuecultureplates.

CauseofDifferation:ConcentrationofEScells»Answer

Recommendation:LowconfluencyofEScellscanresultindifferentiation.EScellsshouldbeplatedataminimumdensityof1x106cells/100mmdish.RefertotheattachedfiguresforillustrationsofEScellconfluency.Refertofigs.1,2,3,4,and5.

CauseofDifferation:Incubatorsettings»Answer

Recommendation:EnsurethattheCO2incubatorreADIngsarecorrectat37oCand5%CO2.

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MSDS
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MSDS
- ProductthatdoesnotrequireanMSDS
Protocols
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Protocols
- ElectroporationofESCells
- SelectionofESCells
- HarvestingandDNAPreparation
- ESCellCultureusingESGROMediumSupplement
- ColonyPicking
- KaryotypingESCells
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References
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References
- High-throughputtrappingofsecretorypathwaygenesinmouseembryonicstemcells.
  De-Zolt,Silke,etal.(2006)NucleicAcidsRes.,34:e25(2006)
- EnforcedactivationofSTAT5Afacilitatesthegenerationofembryonicstem-derivedhematopoieticstemcellsthatcontributetohematopoiesisinvivo.
  Schuringa,JanJacob,etal.(2004)StemCells,22:1191-204(2004)
- Establishmentincultureofpluripotentialcellsfrommouseembryos
  Evans,M.J.andKaufman,M.(1981)Nature,292:154-156(1981)
- DifferentiationofMouseStemCellsinCollagenTypeIGels
  Ahsan,etal.(2004)Regenerate2004,SeattleWashington,POO1:(2004)
- Clonalanalysisoftheactionsofthemurineleukaemiainhibitoryfactoronleukemicandnormalmurinehaemopoieticcells
  MetcalfD.,HiltonDJ.,andNicolaNA.(1988)Leukemia,2:216-221(1988)
- Inhibitionofpluripotentialembryonicstemcelldifferentiationbypurifiedpolypeptides
  SmithAG.,HeathJK.,andDonaldsonDD.(1988)Nature,336:688-690(1988)
- Myeloidleukemiainhibitoryfactormaintainsthedevelopmentalpotentialofembryonicstemcells
  WilliamsRL.,HiltonDJ.,andPeaseS.(1988)Nature,336:686-687(1988)
- Isolationofapluripotentcelllinefromearlymouseembryosculturedinmediumconditionedbyteratocarcinomastemcells
  Martin,G.R.(1981)Proc.Natl.Acad.Sci.USA,78:7634-7638(1981)
- Formationofaprimitiveectodermlikecellpopulation,EPLcells,fromEScellsinresponsetoBIOLOGicallyderivedfactors
  Rathjen,J.etal.(1999)JournalofCellScience,112:601-612(1999)
- Cultureanddifferentiationofembryonicstemcells
  Smith,A.G.(1991)J.TissueCultureMethods(nowMethodsinCellScience),13:89-94(1991)
- DifferentiationofRadialGlia-LikecellsfromembryonicStemCells
  Liour,SandYu,RK.(2003)GLIA,42(2):109-117(2003)
- Theoriginandefficientderivationofembryonicstemcellsinthemouse
  Brook,F.A.andGardner,R.L.(1997)Proc.Natl.Acad.Sci.USA,94:5709-5712(1997)
- Germ-linetransmissionofadisrupted(2-microglobulingeneproducedbyhomologousrecombinationinembryonicstemcells
  ZijlstraM,LiE,SajjadiF,SubramaniS,andJaenischR.(1989)Nature,342:435-438(1989)
- Establishmentofgermlinecompetentembryonicstem(ES)cellsusingdifferentiationinhibitingactivity
  Nichols,J.,Evans,E.P.andSmith,A.G.(1990)Development,110:1341-1348(1990)
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TechnicalLiterature
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TechnicalLiterature
- Protocol:PrimaryEmbryonicFibroblastCulture
- StemCellTestingofPESMembraneContainingStericupFilters
- Protocol:ESStemCellTroubleshootingGuide
- Protocol:Reagents
- Protocol:KaryotypingESCells
- Protocol:ESCellCulture
- Protocol:IsolationofPrimaryMouseEmbryonicFibroblasts(PMEFs)
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ESGRO® (LIF) ESG1106

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