ESGRO®(LIF) ESG1107

TradeName:Chemicon(Millipore)

KeyApplications:StemCellCulture

ApplicationNotes:ApplicationsforESGRO®includeuseasareagentfortheinvitromaintenanceofthepluripotentialphenotypeofmurineEScells.

SUGGESTEDPROTOCOLS:ESCells:InD3andMBL-1pluripotentialEScellculturesitisroutinelyfoundthat1000UofESGRO®per1.0mLoftissueculturemediaisrequiredtomaintainEScellswithastemcellphenotype.SimilarconcentrationsofmLIFhavealsobeenusedforgermlinetransmissionofgeneticallyalteredEScells(E3).

Attherecommendedconcentration10⁷unitsofESGRO®issufficientfor10.0Loftissueculturemediaand10⁶unitsofESGRO®issufficientfor1.0Loftissueculturemedia.

Visitwww.esgro-lif.comforadditionalinformation

SpeciesReactivity:Mouse

UsageStatement:Unlessotherwisestatedinourcatalogorothercompanydocumentationaccompanyingtheproduct(s),ourproductsareintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.

Species:Mouse

CellCultureMediaorReagentType:ESGROSupplement

StemCellType:

StemCellWorkflowStage:Maintenance&Expansion

MediaType:CellCultureMediaAdditives

Format:Purified

Presentation:ESGRO®issuppliedinliquidformas10⁷UnitsESGRO®in1.0mLofphosphatebufferedsalinewith1%w/vbovineserumalbuminBSAasacarrierforstABIlity.

StorageConditions:ESGRO®isshippedatambienttemperature.Acoldpackmaybeincludedforcustomerrelations,howeveritistrulynotneeded.ExtensivestabilitytestshavebeenperformedwithESGRO®duringdevelopment.Thisproductisstableforatleast18monthsfromthedateofmanufacture,intheconcentratedformordilutedinsteriletissueculturemedia,withnolossofactivityonEScells.ForlongtermstorageitisrecommendedthatESGROconcentratebestoredat4°C.Freeze-thawingwillreducepotency.Itisrecommendedthatpriortouse,ESGRO™shouldbedilutedinsteriletissueculturemediaandaliquotedtoaconvenientconcentration,thenstoredat4°C.Freeze-thawingshouldbeavoided.ESGRO™isstableforaminimumof7daysat37°C,5%CO2incubatorduringthecultureofEScells.

WARRANTY

Thehigheststandardsofqualitycontrolareusedinthemanufactureofthisproduct.Nowarrantyisprovidedthatthesaleoruseoftheproducteitheralone,incombinationwithotherproducts,orintheoperationofanyprocess,willnotinfringepatent,intellectualpropertyoranyotherrightsofthirdparties.Thisproductissuppliedforresearchpurposesonly.Itisnotforhumantherapeuticand/ordiagnosticuse.NOTE:ESGRO®shouldnotbeallowedtocomeincontactwithruminantanimalsorswine.



ManufacturedbyCHEMICONInternational,Inc.LIFisprotectedunderUSPatentnos.5,187,077,5,427,925,5,443,825,5,750,654and6,261,548,EuropeanPatentno.0285448andrelatedforeignpatentsandisnotavailableforresale.UseofrecombinantLIF,orCHEMICONsproprietaryESGRO®formulation,forthemaintenanceofembryonicstemcellsisprotectedworld-wideunderthefollowingpatents:US5,166,065,EP380646,andothers.

SpecificActivity:ESGRO®isassessedbothonES{embryonicstem}cells(D3&MBL-1)andonmurineM1myeloidleukemiccells(4).Astandardof50UnitsisdefinedastheconcentrationofESGRO®,in1.0mLoftissueculturemedium,thatinducesthedifferentiationof50%ofM1colonies(4).10E7unitsisequivalenttoapproximately100μgofpureprotein,andissufficienttotreat10LofEScellculturemedia.

EmbryonicStemCellAssay:Differentiationinhibitionat1000units/mLMurinemyeloidleukemic,M1Assay:SpecificActivity>/=10⁸units/mg

Packaging:1mL

CellCultureReagentType:

GeneSymbol:

ProteinorEnzymeType:Cytokines&GrowthFactors

AlternateNames:MurineLIF

Concentration:10millionunits/1mL

Purity:TheactivecomponentmLIFhasbeenshowntobe>95%purebySDS-PAGE.ESGRO®issupplied0.22micronsterilefiltered,andtestednegativeformycoplasmas.