lumafluor Red Retrobeads 产品说明书文章来源：工业电气网 作者：【未知】 点击：次 时间：2016/1/14 20:50:09 [lumafluor Red Retrobeads 产品说明书]:上海起福生物科技有限公司专业代理lumafluor全线产品 。并被有大量现货，欢迎广大科研人员订购联系电话 4006551678订购信息 Item Price Red Retrobeads （10...immersion objective （numerical aperture of 0.4 or greater）, or higher power dry objectives, will usually reveal the cells. However, frequently a X25 immersion objective will reveal very clearly labeled cells that lower power objectives miss. These caveats are especially true for green beads. Before deciding that an experiment did not work, examine sections in the vicinity of the injection site with immersion objectives. Numerous locally labeled cells should be present.
Additional information for green bead users: In work that has been done so far, it appears that younger animals transport the label better than older animals. In addition, tissue autofluorescence is lower in the younger animals. Therefore, it is advisable to use younger animals, if possible, in experiments involving green beads.
Because the green beads are excited at shorter wavelengths than red beads, tissue autofluorescence is a greater problem. Therefore, efforts to minimize autofluorescence will produce a better contrast signal. Ways to reduce autofluorescence include: 1） using thinner sections （e.g. 30 um rather than 40 or 50） 2） using younger animals, and 3） examining sections promptly after coverslipping （background increases over time）.
InformationFor Reliable, Robust Retrograde Transport, There\"s Only One Choice: RetroBeads from Lumafluor.
RetroBeads from Lumafluor--the original microspheres for retrograde tracing and the only microspheres proven effective where it counts: in your experiments. Green and Red fluorescent RetroBeads are available exclusively from Lumafluor.
Do not be misled by the unsubstantiated claims of other suppliers!
No matter what they call their products, only Retrobeads from Lumafluor have been proven effective by hundreds of published papers in systems ranging from invertebrates to primates, and everything in between. Lumafluor Retrobeads : Highly confined injections--superb for detailed connectivity studies. Persist indefinitely （ 1 year!） in living cells, nontoxic. Compatible with most other anterograde tracers, in situ hybridization, and immunohistochemsitry.
Retrosphere Color: Excitation Max （nm） Emission Max （nm） Green 460 505 Red 530 590
New!
Retrobeads IX: Retrobeads deliver bioactive agents （such as neurotrophins and neurotransmitter agonists/antagonists） to localized regions; retrograde transport allows determining which neurons were exposed to the agents [Riddle et al. Nature 378:189, 1995 and Quattrochi et al. Science 245:984, 1989]. Retrobeads IX are specially prepared to facilitate adsorption of proteins and other bioactive compounds. Retrobeads IX are also more effective tracers in primate systems than standard Retrobeads . 123
