Megazyme’scatalaseassaykitprovidesasimplecolourimetricmethodforthemeasurementofcatalaseactivity.Themethodisdeliveredinafastandreliableformatandmaybeusedtodetectcatalaseactivityinvarioussamples,includingfood,BIOLOGicalandbacterialsamples.ThecalculationmethodincludedallowsforsignificantvariationintheconcentrationoftheH2O2substratesolutionthatisemployedintheassaywhichtranslatesintoareductioninthe‘handson’timerequiredbytheanalystincomparisontoothercommercialkits.
Colourimetricmethodforthedeterminationof catalaseactivity.
Principle:
(catalase)
(1)2H2O2 → H2O + O2
(peroxidase)
(2) 2H2O2 +DHBS +AAP → quinoneimine +4H2O
(purplecolour)
Note:
DHBS = 3,5-Dichloro-2-hydroxybenzenesulfonicacid
AAP=4-aminoantipyrene
Kitsize: 100/200assays
Method: Spectrophotometricat520nm
Totalassaytime: 20min
Detectionlimit: 0.5U/mL
Applicationexamples:Variousfood,biologicalandbacterialsamples
Methodrecognition: Novelmethod
Advantages