Megazyme/αNAcetylgalactosaminidase (microbial) /EANAGM/2 Units at 37^oC

DESCRIPTION

α-N-Acetylgalactosaminidase(microbial)

EC 3.2.1.49
CAZyFamily:GH36
CAS: 9075-63-2

Synonyms:
alpha-N-acetylgalactosaminidase;alpha-N-acetyl-D-galactosaminideN-acetylgalactosaminohydrolase

Form:
Insolution(Tris.HCl/NaCl/EDTA).

Stability:
>2yearsat4^oC.

Specificactivity:
~7.0U/mg(37^oC,pH7.5on pNP-α-D-N-acetylgalactosamine).

Unitdefinition:
OneUnitofα-N-acetyl-D-galactosaminidaseactivityisdefinedastheamountofenzymerequiredtoreleaseoneμmoleofp-nitrophenolperminutefrompNP-α-D-N-acetylgalactosamine(1mM)inTris.HClbuffer(100mM),pH7.5at37^oC.

Specificity:
Cleavageofnon-reducingα-(1,3)-N-acetylgalactosamineresiduesfromhumanbloodgroupAandABmucinglycoproteins,ForssmanhaptenandbloodgroupAlactoseriesglycolipids.

Applications:
Applicationsinclinicalchemistry,glycoBIOLOGyandcarbohydrateresearch.