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Highpurityβ-N-Acetylhexosaminidase(prokaryote)foruseinresearch,biochemicalenzymeassaysand invitro diagnosticanalysis.
EC3.2.1.52
CAZyFamily:GH20
CAS:9012-33-3
beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase
Recombinant.Fromaprokaryote.
In3.2Mammoniumsulphate.
Suppliedat~1,000U/mL.
Specificactivity:
~260U/mgprotein(on pNP-β-D-N-acetylglucosamine)atpH4.0and40oC;
~723U/mgprotein(on pNP-β-D-N-acetylglucosamine)atpH4.0and60oC.
StABIlity: >2yearsat4oC.
DESCRIPTION
β-N-Acetylhexosaminidase(prokaryote)
EC3.2.1.52
CAZyFamily:GH20
CAS:9012-33-3
Synonyms:
beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase
Form:
In3.2Mammoniumsulphate.
Stability:
>2yearsat4oC.
Specificactivity:
~260U/mgprotein(on pNP-β-D-N-acetylglucosamine)atpH4.0and40oC;
~723U/mgprotein(on pNP-β-D-N-acetylglucosamine)atpH4.0and60oC.
Unitdefinition:
OneUnitofpyruvatekinaseactivityisdefinedastheamountofenzymerequiredtoconvertoneµmoleofphosphoenolpyruvatetopyruvatepermininTris.HClbuffer(100 mM)atpH7.2at37oC.
Specificity:
Hydrolysisofterminalnon-reducing N-acetyl-D-hexosamineresiduesin N-acetyl-β-D-hexosaminidesfromglycoproteinsandoligosaccharides.
Applications:
ForuseinglycoBIOLOGyresearch.