Megazyme/Wheat Arabinoxylan (acid debranched, 26% Ara)/PADWAX26/3 grams

HighpurityWheatArabinoxylan(aciddebranched;26%Ara)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.

Preparedbycontrolledacidhydrolysisofwheatflourarabinoxylan.

Purity>94%.Ara:Xyl=26:74.Glucose,galactoseandmannose<2%.

AComparisonofPolysaccharideSubstratesandReducingSugarMethodsfortheMeasurementofendo-1,4-β-Xylanase

McCleary,B.V.&McGeough,P.(2015).Appl.Biochem.Biotechnol.,177(5),1152-1163.

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Themostcommonlyusedmethodforthemeasurementofthelevelofendo-xylanaseincommercialenzymepreparationsisthe3,5-dinitrosalicylicacid(DNS)reducingsugarmethodwithbirchwoodxylanassubstrate.ItiswellknownthatwiththeDNSmethod,muchhigherenzymeactivityvaluesareobtainedthanwiththeNelson-Somogyi(NS)reducingsugarmethod.Inthispaper,wehavecomparedtheDNSandNSreducingsugarassaysusingarangeofxylan-typesubstratesandaccuratelycomparedthemolarresponsefactorsforxyloseandarangeofxylo-oligosaccharides.Purifiedbeechwoodxylanorwheatarabinoxylanisshowntobeasuitablereplacementforbirchwoodxylanwhichisnolongercommerciallyavailable,anditisclearlydemonstratedthattheDNSmethodgrosslyoverestimatesendo-xylanaseactivity.UnliketheDNSassay,theNSassaygavetheequivalentcolourresponsewithequimolaramountsofxylose,xylobiose,xylotrioseandxylotetraosedemonstratingthatitaccuratelymeasuresthequantityofglycosidicbondscleavedbytheendo-xylanase.TheauthorsstronglyrecommendcessationoftheuseoftheDNSassayformeasurementofendo-xylanaseduetothefactthatthevaluesobtainedaregrosslyoverestimatedduetosecondaryreactionsincolourdevelopment.

WheatArabinoxylan(aciddebranched,26%Ara)
CAS:9040-27-1

Preparation:
Preparedbycontrolledacidhydrolysisofwheatflourarabinoxylan.

Stability:
>5yearsatroomtemperature

Properties:
Purity:>94%(dwbasis).Glucose,galactoseandmannose<2%
SugarRatio:Arabinose:Xylose=26:74
Viscosity:2.0cSt(1%w/v;OstwaldC-typeviscometer,30^oC)
Starch:<0.2%
Beta-Glucan:<0.1%
Protein:2.3%
Moisture:2.2%
Ash:2.3%
PhysicalDescription:Slightlyoff-white,odourlesspowder

Applications:
Potentialapplicationsinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.

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Megazyme/Wheat Arabinoxylan (acid debranched, 26% Ara)/PADWAX26/3 grams

AComparisonofPolysaccharideSubstratesandReducingSugarMethodsfortheMeasurementofendo-1,4-β-Xylanase