D葡萄糖【GOPOD法】检测试剂 DGlucose (GOPOD Format) Assay Kit 产品货号：KGLUC

货号：

K-GLUC

产品货号：K-GLUC

品牌：Megazyme

产品品名：D-葡萄糖【GOPOD法】检测试剂盒

英文品名：D-Glucose(GOPODFormat)AssayKit

规格型号：660assaysperkit

HighpurityreagentforassayofD-glucoseincerealextractsandforuseincombinationwithotherMegazymekits.

INTRODUCTION:

D-Glucosecanbeconvenientlymeasuredinbodyfluidsusingcommerciallyavailablekitsbasedontheglucoseoxidase/peroxidaseoronthehexokinase/G6P-DHenzymicprocedures.However,
D-glucoseinplantextractsusuallyoccurstogetherwithmaltose,maltosaccharides,starch,sucroseand/orβ-linkedgluco-oligosaccharides.Consequently,morestringentrequirementsareplacedonthepurityoftheassayreagents.Thereagentsmustbeessentiallydevoidofstarchdegradingenzymes,sucrosedegradingenzymesandβ-glucosidase,asthesecanleadtoeitheranover-estimationoranunder-estimationoffreeD-glucosepresentintheextractorderivedbyspecificenzymicdegradationofD-glucosecontainingoligosaccharidesorpolysaccharides(e.g.barleyβ-glucan).MostcommerciallyavailableD-glucosekitsbasedontheglucoseoxidase/peroxidasereactioncontainreagentswhicharenotsufficientlypure.
TheMegazymeD-Glucose(glucoseoxidase/peroxidase;GOPOD)AssayKitemployshighpurityglucoseoxidaseandperoxidaseandcanbeusedwithconfidenceforthespecificmeasurementofD-glucoseinextractsofplantmaterialsorfoods.Thecolourwhichformsisstableatroomtemperatureforatleasttwohoursafterdevelopment.

KITS:

Kitssuitableforperforming660assays(3mLperassay)areavailablefromMegazyme.Thekitscontainthefullassaymethodplus:
Bottle1:(x2)GOPODReagentBuffer.Buffer(48mL,pH7.4),p-hydroxybenzoicacidandsodiumazide(0.4%w/v).
Stablefor>4yearsat4°C.
Bottle2:(x2)GOPODReagentEnzymes.Glucoseoxidase
plusperoxidase
and4-aminoantipyrine.Freeze-driedpowder.
Stablefor>5yearsat-20°C.
Bottle3:D-Glucosestandardsolution(5mL,1.0mg/mL)in0.2%(w/v)benzoicacid.
Stablefor>5yearsatroomtemperature.

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