产品货号:K-ETSULPH
品牌:Megazyme
产品品名:二氧化硫检测
试剂盒英文品名:TotalSulphite(Enzymatic)AssayKit
规格型号:50assays(manual)/500assays(microplate)/588assays(auto-analyser)
Asimple,highlyspecificandreliable,enzymaticmethodforthemeasurementoftotalsulphite(asSO2)inwines,beverages,foodstuffsandothermaterials.Suitableformanual,auto-analyserandmicroplateformats.
INTRODUCTION:
Sulphurdioxide(SO2)isusedwidelyasanadditiveinvariousforms,most
commonlyassulphites(orsulfites),inthewine,beverageandfoodindustries
whereitactsprimarilyasanantimicrobialandantioxidantpreservative.
Duringwinemaking,sulphitesareusedasanessentialadditive,usually
atpost-malolacticfermentation,inthecontrolofcontaminationby
Brettanomycesduringageingandtoalsoprotectthewineagainst
detrimental“oxidativeandenzymaticbrowning”.SO2isonlyactive
asanantimicrobialandantioxidantpreservativeintheunbound
“free”form.GiventhatSO2becomes“inactive”whenitbindsthe
colourpigmentsofwine,andwithlegalrestrictionsonSO2levelsin
wine,ithasbecomevaluabletowineproducerstomeasureboththe
FreeSO2andTotalSO2.Inadditiontothis,duetotheincreased
awarenessoftheadverseeffectsofsulphitesandtheprevalence
ofsulphiteintoleranceinsomeindividuals,sulphitelevelsinfoods
anddrinksarestrictlyregulatedbyvariousgoverningbodiesand
thereforethereisarequirementforaccuratedeterminationofthe
levelofsulphitesinfoods,beveragesandwines.
Thiskit(K-ETSULPH)issuitableforthespecificmeasurementof“Total
Sulphite”asSO2inwines,beverages,foodstuffsandothermaterials.
KITS:
Kitssuitableforperforming50assaysinmanualformat(or588
assaysinauto-analyserformator500assaysinmicroplateformat)are
availablefromMegazyme.Thekitscontainthefullassaymethodplus:
Bottle1:Buffer(28mL;pH8.0)plussodiumazide
(0.02%w/v)asapreservative.
Stablefor>2yearsat4°C.
Bottle2:NADH.
Freezedriedpowder.
Stablefor>2yearsat-20°C.
Bottle3:NADHperoxidasesuspension(1.1mL).
Stablefor>2yearsat4°C.
Bottle4:Sulfiteoxidasesuspension(1.1mL).
Stablefor>2yearsat4°C.
Bottle5:Sodiumsulphitestandardpowder(~5g).
Stablefor>5yearsatroomtemperature.
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温馨提示:不可用于临床治疗。