公司旨在为客户提供高质量的实验外包服务（CRO服务），包括实验方案设计，常规基因工程实验操作，重组蛋白表达与纯化，重组抗体制备，天然蛋白分离与纯化，病毒分离培养等免疫学相关技术服务；同时我们也为工业客户提供部分IVD级别抗体和抗原原料。核心平台：---噬菌体抗体展示技术平台苏州蚂蚁淘生物科技有限公司利用自有的噬菌体（M13，T7噬菌体）展示技术平台，能够为客户提供极具性价比的重组Vhh抗体（纳米抗体/驼类纳米抗体）制备方案，包括动物免疫，文库建立，文库筛选以及重组抗体的体外表达等。---抗体工程技术平台卡梅德生物科技的抗体工程平台包含单克隆抗体制备服务平台，多克隆抗体制备服务平台，抗体测序与改造平台。单克隆抗体制备平台：基于杂交瘤技术的小鼠单抗技术平台，最快能够在2-3个月获得高亲和力与高特异性的单克隆抗体，同时基于噬菌体展示技术，我司能够制备多物种的重组单克隆抗体，包括小鼠，兔子，骆驼，羊驼以及人单抗。多克隆抗体制备服务平台：能够为客户提供兔多抗，羊多抗，骆驼类多抗，牛多抗等多物种的抗体制备服务，根据不同的抗原类型，来自蚂蚁淘生物的资深抗体专家会为您量身定做适合您需求的方案，以满足您的项目要求并节省您的宝贵时间。抗体一级序列测序平台：为满足大量客户对抗体基因序列与氨基酸序列信息的需求，我们为客户提供De Novo抗体全长氨基酸序列解析和N端测序服务；同时我们设计积累多达80种杂交瘤抗体基因测序引物。抗体改造技术平台：蚂蚁淘生物技术的抗体改造平台包括嵌合抗体制备，抗体亲和力成熟以及抗体人源化服务。目前我们已经成功制备十几例嵌合抗体与人源化抗体，均成功交付。---蛋白表达与纯化服务技术平台蚂蚁淘生物科技目前拥有四大蛋白表达平台，能为客户提供全面且优质的蛋白表达技术服务。哺乳动物表达平台：目前公司拥有Freestyle CHO，Expi CHO，CHO-DG44，HEK293F，Vero等细胞系用于蛋白表达制备。公司秉承质量优先原则，全部统一采用Gibco细胞培养基和相关转染配套试剂，以保证每个批次的哺乳动物细胞蛋白表达产品高质量、高表达水平的交付。同时公司拥有的表达载体体系，远高于市面常用蛋白表达载体，能够为客户提供高纯度蛋白。昆虫细胞表达平台：目前公司常采用的昆虫表达细胞为SF9，配套使用的是来自Gibco的培养基，只...

NanoLight

纳米光®科技公司是一家生物技术公司，开发天然的、发光的海洋蛋白质或海洋生物发光的广泛应用。纳米光部门致力于成为制药、农业和研究产品行业中高价值试剂和报告系统的领导者。

NanoLight® Technology is the technology division of Prolume® Ltd., a company focusing upon broad based applications of marine bioluminescence. The NanoLight® division is dedicated to providing scientists with the highest quality reagents, luciferins (NanoFuel®s), recombinant bioluminescent proteins luciferases (NanoLight®s), photoproteins (NanoFlashTM) and fluorescent proteins (NanoFluorsTM).

Bioluminescence

Brief Background onBioluminescence, Luciferases, Photoproteins, Fluorescent Proteins:

Three classes of proteins from bioluminescent organisms have distinctive properties useful as reporter genes for cellular research, in defining metabolic pathways, and extremely useful for high-throughput screening (HTS).

Luciferases catalyze the enzymatic oxidation of a luciferin substrate (notably Coelenterazine), and produce light as a byproduct of this reaction. Unlike first-generation firefly luciferase systems, these Coelenterazine based luciferases (NanoLight™) do not require accessory high energy molecules such as ATP, or Coenzyme A, which greatly simplifies their use in a number of reporter applications.

One notably small, the smallest and THE BRIGHTEST, Coelenterazine luciferase is called Gaussia princeps Luciferase.  It was discovered from many trips to sea to obtain these small 5 mm long copepods that live in sparse distribution at 700-900 meters depth. Obtaining enough of these copepods in pristine condition to make functional libraries for expression cloning was extremelylaborious and expensive.

One application of Gaussia Luciferase is for example the observation of single neuronal vesicle release and thus can be used to image depolarization and the release of insulin and other fusion proteins directly and in a quantitative way.

Photoproteins (Aequorin, Obelin) are “pre-charged” with Coelenterazine and molecular oxygen— when oxidation is triggered by calcium, a flash of light occurs.  Photoproteins usually require a reducing environment and extremely low levels of Calcium otherwise they behave as poor luciferases in the presence of Calcium.

Green Fluorescent Proteins (NanoFluor™) intrinsically fluoresce as a result of a unique peptide-derived chromophore that self-assembles after translation. GFPs’ ability to shift the wavelength of luciferase or photoprotein from blue to green allows, among other uses, whole cell studies of gene expression and the visualization of drug-target interactions over time & at variable dosages.

Our unique GFP and luciferase properties enabled company scientists to obtain a Phase I SBIR to evaluate the feasibility of BRET (bioluminescent energy transfer) for use as a novel class of biosensors. BRET is potentially a broadly applicable enabling technology for HTS, medical diagnostics and basic research. Its singular advantage is that it provides both spatial and orientation information when a binding or proximity event occurs.

One notable sea pen GFP,Ptilosarcus, was found to have has the highest natural quantum efficiency of all fluorescent proteins. Ptilosarcus and Renilla GFP have been codon optimized and used in High Content Screening by Cellomics, and in use by Molecular Devices, and Rigel Pharmaceuticals.

NanoLight Technology believes the basic researcher, drug discoverer, and agrichemical industries are only beginning to harness the power of naturally occurring bioluminescence in their research, screening, and discovery efforts.

Most of the visible spectrum measurable by standard luminometers remains available for exploitation, NanoLights™ are well-positioned to develop bioluminescent reporter systems, and easily incorporated into ELISA detection systems, direct in vivo imaging, cell tracking, and in DNA-DNA, DNA-RNA, and nucleotide-peptide detection using a variety of strategies, and split luciferase techniques.  Because Intellectual Property Rights using NanoLight Technology’s bioluminescent technology are solid and unencumbered by prior art, life science companies have assurance that their discoveries are safe and subsequent patent positions based upon NanoLight Technology are secure.

苏州蚂蚁淘生物科技有限公司为NanoLightX品牌在华授权代理商，
公司不仅坚持提供传统标记的二抗（如酶标记，AMCA，FITC，罗丹明，Cy系列等），
而且始终与*的染料生产商合作，以最快的速度供应新型染料标记的二抗（如Alexa Fluor，DyLight 405）。
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