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Nanolight/Prolume Purple/3691 ( 2 x500ug)/1 mg

Prolume Purple or methoxy e-Coelenterazine (Me-O-e-CTZ) is a new synthetic analogue of e-Coelenterazine with an additional methoxy group quenching the emission maximum from 475 nm to 405 nm. Nanolight™ Technologies developed Prolume Purple. (US Patent 2013/057660)

Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).
Please see our
"How to use" for further information. 

 

Compound ID: Prolume Purple (PP-CTZ)
alternative name: methoxy e-Coelenterazine (me-O-eCTZ)
Molecular Formula: C29H25N3O3
MW: 463.53
Structure:
Application: emits around 405 nm with RLuc, RLucII, RLuc3, RLuc8 and Green Renilla Luc
Solvent: We recommend NanoFuel Solvent. Low solubility in Ethanol.
comparison to CTZ400a:  
Emission spec with Renilla Luciferases:  
References:

Bioluminescence Assisted Switching and Fluorescence Imaging (BASFI)
Luyuan Zhang, Fang Xu, Zhixing Chen, Xinxin Zhu and Wei Min. 
J.Phys. Chem. Lett., 2013, 4, pp 3897-3902

Prolume Purple (Cat. #369) was enabling these researches to follow protein movement within a single cell in real-time for up to 20 min using a sophisticated BRET system:

Monitoring G protein-coupled receptor and beta-arrestin trafficking in live cells using enhanced bystander BRET

Yoon Namkung, Christian Le Gouill, Viktoria Lukashova, Hiroyuki Kobayashi, Mireille Hougue, Etienne Khoury, Mideum Song, Micharl Bouvier, Stephane A. Laporte

 

How to use Prolume Purple

Certificate of Analysis    HPLC     MSDS     NMR

The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 250 ug and 500 ug aliquots.
1 mg = 2x 500 ug. 10 mgs is packed as regular powder in one tube.

The advantages of lyophilized luciferins are:

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packed under Argon)

 


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